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Superdex 75 10 300 sec

Manufactured by GE Healthcare
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The Superdex 75 10/300 SEC is a size-exclusion chromatography column designed for the purification and analysis of proteins, peptides, and other biomolecules. It is suitable for a wide range of molecular weight separations, from approximately 3 kDa to 70 kDa. The column features a high-resolution, high-flow design that enables efficient and reproducible separations.

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Lab products found in correlation

Kta fplc system, by GE Healthcare (1 mentions) Omnisec software, by Malvern Panalytical (1 mentions) Akta avant, by GE Healthcare (1 mentions) Minidawn treos, by Wyatt Technology (1 mentions) Optilab t rex, by Wyatt Technology (1 mentions) Astra software, by Wyatt Technology (1 mentions)

2 protocols using superdex 75 10 300 sec

1

SEC-SLS Analysis of AKAP18β-RIIα Complex

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Static light scattering (SLS) experiments were carried out using a Superdex 75 10/300 SEC analytical column attached to an ÄKTA FPLC system (both GE Healthcare), coupled to VE3580 RI and 270 Dual detectors (Viscotek). Evaluation of the data was performed using the OmniSec software (Viscotek) and the RI signal. The protein concentration of the complex AKAP18β(43–83)–RIIα-D/D(1–44) was adjusted to 200 μM in 20 mM Hepes, 150 mM NaCl, pH 7.5, complemented with 2 mM CdCl2 or 2 mM EDTA. All samples were injected in 200-μl aliquots. The Superdex 75 10/300 column was equilibrated with the same buffer, and run at a flow rate of 0.5 ml/min.
Götz F., Roske Y., Schulz M.S., Autenrieth K., Bertinetti D., Faelber K., Zühlke K., Kreuchwig A., Kennedy E.J., Krause G., Daumke O., Herberg F.W., Heinemann U, & Klussmann E. (2016). AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA. The Biochemical journal, 473(13), 1881-1894.
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2

Size Exclusion Chromatography and MALS

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The size exclusion chromatography (SEC) was performed using a Superdex 75 10/300 SEC column operated by AKTA Avant (GE Healthcare). The multi-angle light scattering (MALS) was performed with miniDAWN TREOS (WYATT Technology) and its companion Optilab T-rEX (WYATT Technology, Santa Barbara, CA, USA) dRI detector. The analysis of the SEC-MALS data was performed using the ASTRA software (WYATT Technology).
Mayer M., Matiuhin Y., Nawatha M., Tabachnikov O., Fish I., Schutz N., Dvir H, & Landau M. (2021). Structural and Functional Insights into the Biofilm-Associated BceF Tyrosine Kinase Domain from Burkholderia cepacia. Biomolecules, 11(8), 1196.
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