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Wt1 antibody

Manufactured by Abcam
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The WT1 antibody is a tool used in scientific research to detect and study the Wilms' Tumor 1 protein. WT1 is a transcription factor involved in the regulation of gene expression during embryonic development and in some adult tissues. The antibody can be used to identify the presence and localization of WT1 in various cell and tissue samples through techniques such as immunohistochemistry and Western blotting.

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Lab products found in correlation

Puromycin aminonucleoside, by Merck Group (1 mentions) Rna extraction kit, by Takara Bio (1 mentions) Alexa fluor 647 annexin 5 and propidium iodide, by BioLegend (1 mentions) Gapdh, by Proteintech (1 mentions)

5 protocols using wt1 antibody

1

Quantitative Analysis of Podocytes

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Immunofluorescent analysis of mouse kidney sections was carried out as described above using a WT‐1 antibody (Abcam). 4′,6‐diamidino‐2‐phenylindole (DAPI) was used to counterstain nuclei. ImageJ software was used to count WT‐1 positive nuclei and expressed as a percentage of the total number of DAPI stained cells per glomerulus.
Hurcombe J.A., Lay A.C., Ni L., Barrington A.F., Woodgett J.R., Quaggin S.E., Welsh G.I, & Coward R.J. (2019). Podocyte GSK3α is important for autophagy and its loss detrimental for glomerular function. FASEB bioAdvances, 1(8), 498-510.
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2

Comprehensive Molecular Expression Analysis

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The antibodies against MCC, CD2AP, total β-catenin, p16, p21, p27, p57, CDK1, Cyclin E1, Cyclin D2, PCNA, and GAPDH (Proteintech, USA); antibodies against active β-catenin, total ERK1/2 and phospho-ERK1/2, total AKT and phospho-AKT (Cell Signaling Technologies, USA); WT1 antibody (Abcam, USA); puromycin aminonucleosides (Sigma, USA); Alexa Fluor® 647 Annexin V and Propidium Iodide (Biolegend); Reverse transcription kit, DRR037A (Takara); quantitative PCR kit (Thermo Fisher Scientific); RIPA cell lysis buffer, BCA protein quantification kit (Beyotime, Shanghai); RNA extraction kit (Takara).
Song H., Zhuang L., Xu X., Shi J., Hu W., Liu Z, & Shi S. (2021). MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene. Frontiers in Medicine, 8, 777563.
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3

Quantifying Podocyte Numbers in Mouse Kidney

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Immunofluorescent analysis of mouse kidney sections was carried out as described above using a WT-1 antibody (Abcam). 4′,6-diamidino-2-phenylindole (DAPI) was used to counterstain nuclei. Image J software was used to count WT-1 positive nuclei and expressed as a percentage of the total number of DAPI stained cells per glomerulus.
Hurcombe J.A., Lay A.C., Ni L., Barrington A.F., Woodgett J.R., Quaggin S.E., Welsh G.I, & Coward R.J. (2019). Podocyte GSK3α is important for autophagy and its loss detrimental for glomerular function. FASEB bioAdvances, 1(8), 498-510.
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4

Histological Analysis of Peritoneal Tissues

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Kidney, stomach, diaphragm, peritoneum, spleen, intestines, liver, and reproductive tissues in the peritoneal cavity were collected from experimental animals, immediately placed in 10% formalin, and subsequently embedded in paraffin. Slides were made and stained with hematoxylin and eosin (H&E) or WT1 antibody (Abcam). Two different lots of WT1 antibody were used and both lots were first titrated to determine the optimal dilution to use for study samples, which was found to be 1:250 and 1:300. Two board-certified pathologists reviewed H&E and WT1-stained tissues for the presence of hyperplasia and/or MM.
Larson D., Powers A., Ambrosi J.P., Tanji M., Napolitano A., Flores E.G., Baumann F., Pellegrini L., Jennings C.J., Buck B.J., McLaurin B.T., Merkler D., Robinson C., Morris P., Dogan M., Dogan A.U., Pass H.I., Pastorino S., Carbone M, & Yang H. (2016). Investigating palygorskite’s role in the development of mesothelioma in southern Nevada: Insights into fiber-induced carcinogenicity. Journal of Toxicology and Environmental Health. Part B, Critical Reviews, 19(5-6), 213-230.
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5

Histological Analysis of Peritoneal Tissues

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Kidney, stomach, diaphragm, peritoneum, spleen, intestines, liver, and reproductive tissues in the peritoneal cavity were collected from experimental animals, immediately placed in 10% formalin, and subsequently embedded in paraffin. Slides were made and stained with hematoxylin and eosin (H&E) or WT1 antibody (Abcam). Two different lots of WT1 antibody were used and both lots were first titrated to determine the optimal dilution to use for study samples, which was found to be 1:250 and 1:300. Two board-certified pathologists reviewed H&E and WT1-stained tissues for the presence of hyperplasia and/or MM.
Larson D., Powers A., Ambrosi J.P., Tanji M., Napolitano A., Flores E.G., Baumann F., Pellegrini L., Jennings C.J., Buck B.J., McLaurin B.T., Merkler D., Robinson C., Morris P., Dogan M., Dogan A.U., Pass H.I., Pastorino S., Carbone M, & Yang H. (2016). Investigating palygorskite’s role in the development of mesothelioma in southern Nevada: Insights into fiber-induced carcinogenicity. Journal of Toxicology and Environmental Health. Part B, Critical Reviews, 19(5-6), 213-230.
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