Brains from eight WT and eight Celf6 mutant mice (see) were extracted, frozen in liquid nitrogen, and crushed into a fine powder, from which RNA was extracted using QIAGEN RNEasy columns on a Qiacube robot, following manufacturer’s protocol. RNA was DNase I treated, and RNA quantity and integrity were confirmed using Agilent Bioanalyzer. cDNA was prepared and chemically labeled with Kreatech ULS RNA labeling kit (Kreatech Diagnostics) and Cy5-labeled cDNAs were hybridized to Agilent Mouse v2 4×44K microarrays (G4846A-026655). Hybridization of the labeled cDNAs was done in Agilent 2x gene expression hybridization buffer, Agilent 10x blocking reagent and Kreatech Kreablock onto Agilent 4×44K V2 microarrays at 65°C for 20 min. Slides were scanned on an Agilent C-class Microarray scanner. Gridding and analysis of images was performed using Agilent Feature Extraction V11.5.1.1.
Agilent 4 44k v2 microarrays
Manufactured by Agilent Technologies
The Agilent 4x44K V2 microarrays are high-density gene expression microarrays designed for comprehensive genome-wide analysis. The arrays contain approximately 44,000 probes, allowing for the analysis of a large number of genes simultaneously.
Automatically generated - may contain errors
2 protocols using agilent 4 44k v2 microarrays
1
Transcriptome Analysis of Celf6 Mutant Mice
2
Transcriptome Analysis of Celf6 Mutant Mice
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Brains from eight WT and eight Celf6 mutant mice (see) were extracted, frozen in liquid nitrogen, and crushed into a fine powder, from which RNA was extracted using QIAGEN RNEasy columns on a Qiacube robot, following manufacturer’s protocol. RNA was DNase I treated, and RNA quantity and integrity were confirmed using Agilent Bioanalyzer. cDNA was prepared and chemically labeled with Kreatech ULS RNA labeling kit (Kreatech Diagnostics) and Cy5-labeled cDNAs were hybridized to Agilent Mouse v2 4×44K microarrays (G4846A-026655). Hybridization of the labeled cDNAs was done in Agilent 2x gene expression hybridization buffer, Agilent 10x blocking reagent and Kreatech Kreablock onto Agilent 4×44K V2 microarrays at 65°C for 20 min. Slides were scanned on an Agilent C-class Microarray scanner. Gridding and analysis of images was performed using Agilent Feature Extraction V11.5.1.1.
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