Anti-mouse CD4 (GK1.5), anti-mouse CD8a (53-6.7), anti-mouse CD103 (2E7), anti-mouse CD127 (A7R34), anti-mouse CD11b (M1/70), anti-mouse granzyme C (SFC1D8), anti-mouse Notch1 (HMN1-12) and anti-mouse Notch2 (HMN2-35), anti-mouse CD117 (c-kit, ACK2), anti-mouse Ly-6A/E (Sca-1, D7), and anti-mouse TCRδ (GL3) antibodies were purchased from Biolegend (San Diego, CA). Anti-mouse Eomes (Dan11mag) and anti-mouse/human T-bet (4B10) were purchased from eBioscience (San Diego, CA). Anti-mouse CD3ε (145-2C11), anti-mouse PLZF (R17-809) and PE-CF594 Streptavidin (RUO) were from BD Biosciences (San Jose, CA). Anti-mouse NK1.1 (PK136) was from Biolegend (San Diego, CA) and eBioscience (San Diego, CA). Mouse lineage cell detection cocktail-biotin (#130-092-613) is purchased from Miltenyi Biotech.
Anti mouse eomes dan11mag
Manufactured by Thermo Fisher Scientific
The Anti-mouse Eomes (Dan11mag) is a laboratory reagent used for the detection and analysis of the Eomesodermin (Eomes) protein in mouse samples. Eomes is a transcription factor that plays a crucial role in the differentiation and regulation of various cell types, including T cells and natural killer cells. This reagent is designed to specifically recognize and bind to the Eomes protein, allowing researchers to study its expression and function in mouse models.
Automatically generated - may contain errors
2 protocols using anti mouse eomes dan11mag
1
Comprehensive Immunophenotyping of Mouse Cells
2
Murine T Cell Activation Assay
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Iscove's modified Dulbecco's medium (IMDM) was supplemented with 10% (vol/vol) FBS, penicillin/streptomycin, and 50 μM 2-mercaptoethanol (IMDM-10). Fluorescence-conjugated anti-mouse CD8α [53-6.7), TCRVα2 (B20.1), CD25 (PC61), CD69 (H1.2F3), CD122 (TM-β1), CD127 (SB/199), CD44 (IM7), CD62L (MEL-14), Thy1.1 (OX-7), Thy1.2 (58-2.1), CD45.1 (A20), CD45.2 (104), CCR4 (2G12), CCR5 (HM-CCR5), CXCR3 (CXCR3-173), T-bet (4B10), IFN-γ (XMG1.2), TNFα (MP6-XT22), and Annexin V antibodies were purchased from BioLegend; anti-mouse Ki67, KLRG1 (2F1), and pSTAT5 (pY694) antibodies were purchased from BD Biosciences; anti-mouse Eomes (Dan11mag) antibodies were purchased from eBioscience. Cell death was determined by staining cells with 7-AAD or Live/Dead Fixable Violet Dead Cell Stain (Invitrogen). The coding region of Id3 cDNA was amplified from mouse Id3 cDNA (pBS/KS-mId3sp, Addgene) using a forward primer, 5′-CGGGATCCATGAAGGCGCTGAGCCCGGT-3′, and a reverse primer, 5′-CGGAATTCCACGACCGGGTCAGTGGCAA-3′. After digestion with Bam HI and EcoRI, the PCR product was cloned into BamH1 and EcoRI sites of the MIGR1-FLAG Vector [67 (link)]. CA-IKKβ (S177E and S181E) was released from an IKK-2 S177E S181E plasmid (Addgene) and cloned into Not I site of modified MIGR1 retroviral vector.
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