An anti-BRG1 antibody (ab70558) was purchased from Abcam (San Francisco, CA). Anti-γH2AX (05-636) and anti-H2A (07-146) antibodies were obtained from Merck Millipore (CA). An anti-actin antibody (A5441) and MNase were purchased from Sigma Aldrich (St Louis, MO). Puromycin, etoposide (ETO), and 4-hydroxy tamoxifen (4OHT) were obtained from Sigma Aldrich (St Louis, MO).
Anti actin antibody a5441
Manufactured by Merck Group
Sourced in United States
Anti-actin antibody (A5441) is a laboratory reagent used for the detection and analysis of actin, a key structural protein found in eukaryotic cells. This antibody is commonly used in various immunological techniques, such as Western blotting, immunocytochemistry, and immunohistochemistry, to visualize and quantify the presence of actin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
4 hydroxytamoxifen 4 oht, by Merck Group (1 mentions)
Puromycin, by Merck Group (1 mentions)
Mnase, by Merck Group (1 mentions)
Anti γh2ax 05 636, by Merck Group (1 mentions)
Etoposide eto, by Merck Group (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Anti p38 antibody, by Cell Signaling Technology (1 mentions)
Anti phospho p38 9211, by Cell Signaling Technology (1 mentions)
Anti phospho rela, by Cell Signaling Technology (1 mentions)
Anti cyclin e1 11554 1 ap, by Proteintech (1 mentions)
Anti erk1 2 9102, by Cell Signaling Technology (1 mentions)
Sequencing grade trypsin, by Promega (1 mentions)
Anti tubulin antibody t9026, by Merck Group (1 mentions)
Anti p p38 9211, by Cell Signaling Technology (1 mentions)
Peroxidase affinipure goat anti rabbit igg h l secondary antibody, by Jackson ImmunoResearch (1 mentions)
5 protocols using anti actin antibody a5441
1
Chromatin Remodeling Protein Analysis
2
Antibody Staining for Endothelial Markers
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The anti-CCN1 antibody (26689-1) and anti-NOX4 antibody (14347) were purchased from Proteintech (Chicago, IL, USA). The anti-CD31 antibody (3528S) and tubulin (2128s) were purchased from Cell Signaling Technology (Cambridge, MA, USA). The anti-actin antibody (A5441) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The anti-HSP90 antibody (ab13492), anti-ZO-1 antibody (ab59720), and anti-VE-cadherin antibody (ab33168) were purchased from Abcam (Cambridge, MA, USA). Isolectin GS-IB 4 from Griffonia simplicifolia and Alexa Fluor 488 conjugate (I21411) were purchased from Invitrogen (Carlsbad, CA, USA).
3
Protein Expression Analysis by Western Blot
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Total protein was extracted from the cells with protein lysis buffer, and the protein concentration was subsequently measured by Lowry protein assay. Twenty micrograms of total protein from each sample was resolved by 10% or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to a polyvinylidene difluoride membrane (Millipore), blocked in 5% nonfat milk and immunoblotted with various primary antibodies and horseradish peroxidase-conjugated secondary antibodies. Anti-phospho ERK1/2 (9101, 1:1000), anti-ERK1/2 (9102, 1:500), anti-phospho RelA (3033, 1:1000), anti-phospho p38 (9211, 1:500), and anti-p38 antibodies (9212, 1:500) were purchased from Cell Signaling Technology. An anti-cyclin E1 (11554-1-AP, 1:500) antibody was purchased from Proteintech. An anti-GAPDH (KC-5G5, 1:5000) antibody was purchased from Kangchen. An anti-actin antibody (A5441, 1:2000) was purchased from Sigma-Aldrich. Antibody binding was detected using SuperSignal West Pico Chemiluminescent Substrate (Pierce).
4
Proteomic Analysis of Stress Response
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BTD and DiaAlk were homemade; Catalase (C9322), Iodoacetamide (V900335), Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) (678937), and sodium ascobate (A7631) were purchased from Sigma-Aldrich; dithiothreitol (A100281) was purchased from BBI Life Sciences; IPM (EVU111), Light Azido-UV-Biotin (EVU102), and Heavy Azido-UV-Biotin (EVU151) were purchased from KeraFast; CuSO4 (C493-500) was purchased from Thermo Fisher Scientific; Sequencing grade trypsin (V5113) was purchased from Promega. Anti-actin antibody (A5441) (1:500) and anti-tubulin antibody (T9026) (1:500) were purchased from Sigma-Aldrich; Anti-p-eIF2α (3597) (1:1000) and anti-p-p38 (9211) (1:500) antibodies were purchased from Cell Signaling Technology; Anti-PMK-1 antibody (1:500) was a kind gift from Dr. R. Pukkila-Worley at University of Massachusetts Medical School, Worcester, MA58 (link); Anti-PRDX-2 antibody (1:2000) was a kind gift from Dr. E. Veal at Newcastle University Biosciences Institute, Newcastle upon Tyne, UK30 (link).
5
Antibodies for Protein Detection and Quantification
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Tb cryptate-labeled anti-GST antibody (anti-GST-Tb, 61GSTTLB), D2-labeled anti-FLAG M2 antibody (anti-FLAG-D2, 61FG2DLB), D2-labeled anti-GST antibody (anti-GST-D2, 61GSTDLB), Tb cryptate-labeled anti-FLAG M2 antibody (anti-FLAG-Tb, 61FG2TLB), Tb cryptate-labeled anti-His antibody (anti-His-Tb, 61HisTLB), and D2-labeled anti-His antibody (anti-His-D2, 61HisDLB) were purchased from Cisbio Bioassays. Monoclonal anti-FLAG M2-Peroxidase antibody (A8592), anti-GST-Peroxidase antibody (A7340), anti-ACTIN antibody (A5441), and anti-His antibody (SAB1306085) were purchased from Sigma. Peroxidase AffiniPure goat anti-rabbit IgG (H + L) secondary antibody (111-035-003) and Peroxidase AffiniPure goat anti-mouse IgG (H + L) secondary antibody (115-035-003) were purchased from Jackson ImmunoResearch. Anti-TMPRSS2 antibody (ab92323) was purchased from Abcam.
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