Whole blood samples were taken into vacutainer tubes containing 3.2% trisodium citrate (Greiner Bio-One, Stonehouse, UK) and ethylenediaminetetraacetic acid (BD, Oxford, UK). Samples were taken before heparin administration and 30 min after reversal of heparin by protamine sulfate, PPP was prepared by centrifuging samples twice at 1650g before freezing in aliquots at −80°C for testing later. Full blood cell counts were performed on an ABX Pentra DX 120 automated analyser (Horiba Medical, Northampton, UK). The PT, APTT, Clauss fibrinogen and factors II, V, VII, VIII, IX, X, XI, antithrombin, protein C, free protein S and postoperative anti-Xa activity were measured on an ACL 500 Top (Instrumentation Laboratory, Cheshire, UK) automated coagulometer using standard manufacturer protocols and reagents. Factor XIII activity was measured in a flat-bottomed Immulon 2hb 96-well plate (Diagnostica-Stago, Asnières sur Seine, France) using a chromogenic assay kit from Technoclone (Vienna, Austria) and light absorbance was measured using a plate reader (BioTek, Winoosi, Vermont, USA).
Abx pentra dx 120
Manufactured by Horiba
Sourced in France, United Kingdom
The ABX Pentra DX 120 is a compact, fully automated hematology analyzer designed for clinical laboratories. It provides complete blood count (CBC) analysis, including red blood cell, white blood cell, and platelet parameters. The device utilizes flow cytometry technology to deliver accurate and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Trisodium citrate, by Greiner (1 mentions)
Acl top 500, by Werfen (1 mentions)
Bactec 9240, by BD (1 mentions)
Cobas 8000 c502 analyzer, by Roche (1 mentions)
Cd31 pe, by BD (1 mentions)
Cd45ra fitc, by BD (1 mentions)
Cd16 56 pe, by BD (1 mentions)
Cd45 percp clone 2d1, by BD (1 mentions)
Cd3 percp, by BD (1 mentions)
Cd4 pc7, by BD (1 mentions)
Cd45 percp, by BD (1 mentions)
Trucount tube, by BD (1 mentions)
Fc500 flow cytometer, by Beckman Coulter (1 mentions)
Cd3 fitc, by BD (1 mentions)
Cd4 fitc, by BD (1 mentions)
Cd8 pe, by BD (1 mentions)
9 protocols using abx pentra dx 120
1
Coagulation Markers in Heparin Reversal
2
Fasting Venous Blood Analysis
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Fasting venous blood samples were collected in the morning after 8 h of fasting. The assays were performed at the laboratory in Cagri Private Medical Center and Firat University’s Faculty of Medicine using a biochemical analyzer (ABX Pentra DX 120; HORIBA, Ltd.). Hemograms were determined with an autoanalyzer (Coulter® LH 780 hematology system; Beckman Coulter, Inc.). The blood samples were processed within 30 min after blood collection. Baseline NLR was measured by dividing absolute neutrophil count by absolute lymphocyte count.
3
Preparation and Viral Inactivation of Platelet Lysate
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GMP-compliant PL (Mesengen) was prepared and virally inactivated, as previously described [8 (link), 9 (link)]. Briefly, after obtaining informed consent, buffy coats from healthy volunteers were centrifuged and treated with InterSol solution (318 mg Na-citrate 2H2O, 305 mg Na 2-phosphate anhydro, 105 mg Na dihydrogen phosphate 2H2O, 442 mg Na-acetate 3H2O, 452 mg NaCl, 100 mL H2O, Fenwal Inc., Lake Zurich, Illinois) and subsequently with 20–30% human plasma. Potential pathogens were inactivated by using the Intercept Blood System for Platelets (Cerus Corporation, Concord, California, USA). PL was stored at −80°C for 24 hours before thawing at 37°C for 60 minutes. This procedure was repeated three times to enrich the pool of growth factors. Concentration and sterility of the preparation were determined by the haematology analyzer ABX Pentra DX 120 (Horiba ABX, Montpellier, France) and BACTEC 9240 (Becton and Dickinson), respectively. 5 U/mL heparin was added to cell culture media in order to avoid fibrin gel formation.
4
Comprehensive Clinical Data Collection
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Demographic information, epidemiological history, clinical symptoms, comorbid diseases, imaging features, laboratory data, and length of stay were collected through an electronic medical record system. CBC data were determined using an ABX Pentra DX 120 (Horiba Medical, Montpellier, France) hematology analyzer. Biochemical tests were performed with Roche's Cobas 8000 c502 Analyzer (Roche diagnostics; Geneva, Switzerland). Coagulation tests were performed with the Sysmex CS-2500 System coagulation analyzer (Siemens Healthcare Diagnostics, Erlangen, Germany).
5
Comprehensive Immune Cell Profiling
6
Blood Biochemical and Hematological Analyses
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Blood sample was collected from all the patients on the day of presentation at the FMT-HVD. The biochemical tests were done with automatic analyzer CT 600i Wiener lab group apparatus while the hematological tests with the ABX pentra DX 120 HORIBA ABX Magnos bc.
7
Differential Leucocyte Counting in Rats
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Differential count of leucocytes was performed using the equipment ABX Pentra DX 120 (Horiba Medical, Montpellier, France). Blood was collected from rats after 6 h instillation of vehicle or LPS. This equipment uses a Double Hydrodynamic Sequential System technology (Patent of Horiba ABX), which is a combination of Chlorazol black E cytochemistry, focused flow impedance, and light absorbance measurement for blood cell count and 5-part white blood cell differentials78 (link).
8
Hematological Parameters During Vertigo
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MPV, PDW, platelet count, neutrophil count and lymphocyte count in blood samples were measured. Venous blood samples were obtained from antecubital vein at the time of vertigo attack and collected into tubes containing ethylenediaminetetraacetic acid (EDTA) at 9 am. following an overnight fast. To avoid platelet swelling measurements were done shorter than 30 minutes after sampling. An automated blood cell counter was used for these measurements (Horiba ABX Pentra DX 120).
9
Routine Biochemical Analysis Protocol
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Routine laboratory analyses included serum levels of total protein and albumin (colorimetry), determined with an automatic biochemical analyzer (Konelab 6.0, Winer, Argentina).
Hemoglobin levels were determined by photometry, using the ABX Pentra DX120 (Horiba Ltd, Kyoto, Japan). Serum calprotectin (S100A8/A9, MRP8/14) was measured by ELISA (Multiskan FC Microplate Photometer, Thermo Scientific Uniscience) using a commercially available kit (DRG Diagnostics, EIA5111; Springfield, New Jersey, USA). The standard curve ranged from 3.9 to 250 ng/mL. Plasma zinc and copper values were determined by flame atomic absorption spectrophotometry (Varian, model AA200, Melbourne, Australia), at a wavelength of 213.9nm and 327.4nm, respectively. The normal values for plasma zinc and copper were set at 70-140µg/dL and 50-120µg/dL, respectively.
Hemoglobin levels were determined by photometry, using the ABX Pentra DX120 (Horiba Ltd, Kyoto, Japan). Serum calprotectin (S100A8/A9, MRP8/14) was measured by ELISA (Multiskan FC Microplate Photometer, Thermo Scientific Uniscience) using a commercially available kit (DRG Diagnostics, EIA5111; Springfield, New Jersey, USA). The standard curve ranged from 3.9 to 250 ng/mL. Plasma zinc and copper values were determined by flame atomic absorption spectrophotometry (Varian, model AA200, Melbourne, Australia), at a wavelength of 213.9nm and 327.4nm, respectively. The normal values for plasma zinc and copper were set at 70-140µg/dL and 50-120µg/dL, respectively.
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