Small interfering RNAs (siRNAs) targeting VPS9D1-AS1 (si-VPS9D1-AS1) and negative control (NC) siRNA (si-NC) were obtained from GenePharma Technology (Shanghai, China). miR-525-5p mimic and miR-525-5p inhibitor were synthesized by RiboBio Biotechnology (Guangzhou, China) and used to increase and decrease endogenous expression levels of miR-525-5p, respectively. miRNA mimic NC (miR-NC) and NC inhibitor were used as controls. The HMGA1 overexpression plasmid pcDNA3.1-HMGA1 and empty pcDNA3.1 plasmid were designed and generated by Genearray Biotechnology (Shanghai, China). Lipofectamine™ 2000 was used for all cell transfections according to the manufacturer’s protocol.
Mir 525 5p mimic
Manufactured by RiboBio
Sourced in China
The MiR-525-5p mimic is a laboratory reagent designed to functionally mimic the mature microRNA molecule MiR-525-5p. MicroRNAs are small, non-coding RNA molecules that play a role in the regulation of gene expression. The MiR-525-5p mimic can be used in experimental settings to study the biological functions and molecular mechanisms associated with this specific microRNA.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mir 525 5p mimic
1
Silencing VPS9D1-AS1 in Cancer Cells
2
LINC00659 and miR-525-5p Regulation in HUVECs
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Control‐siRNA, LINC00659‐siRNA, inhibitor control, miR‐525‐5p inhibitor, control‐plasmid, LINC00659‐plasmid, mimic control, and miR‐525‐5p mimic were obtained from Ribobio. To explore the effects of LINC00659 and miR‐525‐5p on HUVECs, HUVECs were transfected with control‐plasmid, LINC00659‐plasmid, control‐siRNA, LINC00659‐siRNA, inhibitor control, miR‐525‐5p inhibitor, LINC00659‐siRNA + inhibitor control, LINC00659‐siRNA + miR‐525‐5p inhibitor group, mimic control, miR‐525‐5p mimic, miR‐525‐5p mimic + control‐plasmid, or miR‐525‐5p mimic + Bax‐plasmid with Lipofectamine™ 2000 (Invitrogen) according to the manufacturer's instructions. After 48 h of transfection, the transfection efficiency was measured through reverse transcriptase‐quantitative polymerase chain reaction (RT‐qPCR).
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