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S6entry elispot reader

Manufactured by Cellular Technology

The S6ENTRY ELISpot reader is a compact and automated instrument designed for the detection and analysis of specific immune responses. It is capable of performing quantitative measurements of cytokine-secreting cells using the ELISpot assay technique.

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2 protocols using s6entry elispot reader

1

SARS-CoV-2 Spike and Nucleocapsid Protein-Induced IFN-γ ELISpot Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
After isolation, splenocytes were seeded at a concentration of 5E5 cells/well and incubated for 24 h in the presence or absence of 10 μg/mL of SARS-CoV-2 spike (catalog number S1N-C52H4; Acro Biosystems) or nucleocapsid (catalog number NUN-C5227; Acro Biosystems). Commercially available ELISpot plates for the evaluation of IFN-γ (for mouse: catalogue number MuIFNg [Immunospot; Cellular Technology Limited]; for rabbits: catalog number 3110-4APW-10 [MabTech]) were used. The assay was performed according to the manufacturer’s guidelines. Plates were analyzed using the S6ENTRY ELISpot reader (Immunospot; Cellular Technology Limited).
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2

SARS-CoV-2 Spike and Nucleocapsid Protein-Induced IFN-γ ELISpot Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
After isolation, splenocytes were seeded at a concentration of 5E5 cells/well and incubated for 24 h in the presence or absence of 10 μg/mL of SARS-CoV-2 spike (catalog number S1N-C52H4; Acro Biosystems) or nucleocapsid (catalog number NUN-C5227; Acro Biosystems). Commercially available ELISpot plates for the evaluation of IFN-γ (for mouse: catalogue number MuIFNg [Immunospot; Cellular Technology Limited]; for rabbits: catalog number 3110-4APW-10 [MabTech]) were used. The assay was performed according to the manufacturer’s guidelines. Plates were analyzed using the S6ENTRY ELISpot reader (Immunospot; Cellular Technology Limited).
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