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Coolcube 1 b w digital camera

Manufactured by MetaSystems
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Sourced in Germany

The CoolCube 1 is a black-and-white digital camera designed for laboratory use. It captures high-quality images and provides a core function of digital image acquisition.

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Lab products found in correlation

Provis ax70, by Olympus (4 mentions) Axio imager z2, by Zeiss (4 mentions) Axio imager z2 microscope, by Zeiss (3 mentions) Dp manager imaging software, by Olympus (2 mentions) Dp manager, by Olympus (2 mentions) Bx53 epifluorescence microscope, by Olympus (2 mentions) Ikaros karyotyping platform, by MetaSystems (2 mentions) Ikaros, by MetaSystems (2 mentions) Dp30bw ccd camera, by Olympus (1 mentions) Ikaros software, by MetaSystems (1 mentions) Fluoroshield mounting medium with dapi stain, by Merck Group (1 mentions) Imager z2, by Zeiss (1 mentions) Dp30bw, by Olympus (1 mentions) Dp30bw camera, by Olympus (1 mentions) Provis ax70 microscope, by Olympus (1 mentions) Prime 95b, by Teledyne (1 mentions) Dp30w, by Olympus (1 mentions) Cellsense dimension software, by Olympus (1 mentions) Ikaros karyotyping software, by MetaSystems (1 mentions) Dp30bw digital camera, by Olympus (1 mentions)

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CoolCube 1 (15 citations) CoolCube camera (7 citations)

10 protocols using coolcube 1 b w digital camera

1

Metaphase Microscopic Imaging Protocol

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Giemsa-stained metaphases were captured on a Zeiss Axio Imager Z2 microscope equipped with an automatic Metafer-MSearch scanning platform and a CoolCube 1 b/w digital camera (MetaSystems, Altlussheim, Germany). Metaphases stained with C-banding and in situ hybridization techniques were captured with a Provis AX70 fluorescence microscope equipped with a DP30BW digital camera (Olympus, Tokyo, Japan). All images were acquired in black and white, and later processed using DP Manager imaging software (Olympus, Tokyo, Japan).
Charvát T., Augstenová B., Frynta D., Kratochvíl L, & Rovatsos M. (2022). Cytogenetic Analysis of the Members of the Snake Genera Cylindrophis, Eryx, Python, and Tropidophis. Genes, 13(7), 1185.
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2

Chromosome Analysis and Karyotyping Protocol

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At least 50 metaphase spreads per individual were analyzed to confirm the diploid chromosome number (2n), karyotype structure, and FISH results. Giemsa-stained preparations were analyzed under Axio Imager Z2 microscope (Zeiss, Oberkochen, Germany), equipped with an automatic Metafer-MSearch scanning platform. Photographs of the chromosomes were captured under 100 × objective using CoolCube 1 b/w digital camera (MetaSystems, Altlussheim, Germany). The karyotypes were arranged using Ikaros software (MetaSystems, Altlussheim, Germany). Chromosomes were classified according to their centromere positions,98 modified as metacentric (m), submetacentric (sm), subtelocentric (st), or acrocentric (a). FISH preparations were inspected using an Olympus BX53 epifluorescence microscope (Olympus, Tokyo, Japan), equipped with an appropriate fluorescence filter set. Black-and-white images were captured under 100 × objective for each fluorescent dye with a cooled DP30BW CCD camera (Olympus) using Olympus Acquisition Software. The digital images were then pseudo-colored (blue for DAPI, red for Cy3, green for FITC) and merged in DP Manager (Olympus). Composed images were then optimized and arranged using Adobe Photoshop CS6.
Imarazene B., Du K., Beille S., Jouanno E., Feron R., Pan Q., Torres-Paz J., Lopez-Roques C., Castinel A., Gil L., Kuchly C., Donnadieu C., Parrinello H., Journot L., Cabau C., Zahm M., Klopp C., Pavlica T., Al-Rikabi A., Liehr T., Simanovsky S.A., Bohlen J., Sember A., Perez J., Veyrunes F., Mueller T.D., Postlethwait J.H., Schartl M., Herpin A., Rétaux S, & Guiguen Y. (2021). A supernumerary “B-sex” chromosome drives male sex determination in the Pachón cavefish, Astyanax mexicanus. Current biology : CB, 31(21), 4800-4809.e9.
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3

Giemsa Staining and C-banding for Karyotyping

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The slides were stained with 5% Giemsa solution. Selected metaphases were captured using a Zeiss Axio Imager Z2 (Zeiss, Oberkochen, Germany), equipped with a Metafer-MSearch automatic scanning platform (MetaSystems) and CoolCube 1 b/w digital camera (MetaSystems). Karyograms were prepared using the Ikaros karyotyping platform (MetaSystems). At least 10 metaphases per individual were studied. We performed C-banding stain to detect heterochromatin distribution according to the protocol of Sumner [46 (link)]. The slides were first treated with 0.2 N HCl for 30 min at room temperature, then with prewarmed 5% Ba(OH)2 for 10 min at 45 °C and finally in 2× SSC for 1 h at 60 °C. Subsequently, the slides were washed with distilled water, air-dried and stained with Fluoroshield mounting medium with DAPI stain (Sigma-Aldrich).
Pensabene E., Yurchenko A., Kratochvíl L, & Rovatsos M. (2023). Madagascar Leaf-Tail Geckos (Uroplatus spp.) Share Independently Evolved Differentiated ZZ/ZW Sex Chromosomes. Cells, 12(2), 260.
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4

Karyotyping Species with Unknown Karyotypes

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Metaphases from species with undescribed karyotypes were stained with 8% Giemsa solution. Selected metaphases were captured using a Zeiss Axio Imager Z2 (Zeiss, Oberkochen, Germany), equipped with a Metafer-MSearch automatic scanning platform (MetaSystems, Altlussheim, Germany) and CoolCube 1 b/w digital camera (MetaSystems, Altlussheim, Germany). Karyograms were prepared using the Ikaros karyotyping platform (MetaSystems, Altlussheim, Germany). At least 10 metaphases per individual were studied.
Clemente L., Mazzoleni S., Pensabene Bellavia E., Augstenová B., Auer M., Praschag P., Protiva T., Velenský P., Wagner P., Fritz U., Kratochvíl L, & Rovatsos M. (2020). Interstitial Telomeric Repeats Are Rare in Turtles. Genes, 11(6), 657.
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5

Automated Metaphase Capture and Imaging

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We captured at least 10 metaphases for each studied specimen and applied method, by either a Zeiss Axio Imager Z2 equipped with automatic Metafer-Msearch scanning platform equipped with a CoolCube 1 b/w digital camera (MetaSystems, Altlussheim, Germany) or a Provis AX70 fluorescence microscope equipped with a DP30BW digital camera (Olympus, Tokyo, Japan).
Kostmann A., Kratochvíl L, & Rovatsos M. (2022). ZZ/ZW Sex Chromosomes in the Madagascar Girdled Lizard, Zonosaurus madagascariensis (Squamata: Gerrhosauridae). Genes, 14(1), 99.
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6

Automated Imaging of Biological Specimens

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For each specimen and method, we captured at least 10 images by either a Zeiss Axio Imager Z2 (Zeiss, Oberkochen, Germany) equipped with an automatic Metafer-MSearch scanning platform and a CoolCube 1 b/w digital camera (MetaSystems, Altlussheim, Germany) or an Olympus Provis AX70 fluorescence microscope equipped with a DP30BW digital camera (Olympus, Tokyo, Japan).
Kostmann A., Augstenová B., Frynta D., Kratochvíl L, & Rovatsos M. (2021). Cytogenetically Elusive Sex Chromosomes in Scincoidean Lizards. International Journal of Molecular Sciences, 22(16), 8670.
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7

Karyotype and Chromosomal Analysis

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The Giemsa-stained slides were scanned with an Axio Imager Z2 microscope (Zeiss, Oberkochen, Germany) equipped with Metafer-MSearch scanning platform and a CoolCube 1 b/w digital camera (MetaSystems, Altlussheim, Germany). The karyograms were constructed in the program Ikaros (MetaSystems). The photos of slides stained by DAPI (slides from C-banding and FISH experiments) were taken by a Provis AX70 microscope (Olympus, Tokyo, Japan) equipped with a DP30BW camera (Olympus). The photos were subsequently processed in DP Manager (Olympus).
Augstenová B., Pensabene E., Kratochvíl L, & Rovatsos M. (2021). Cytogenetic Evidence for Sex Chromosomes and Karyotype Evolution in Anguimorphan Lizards. Cells, 10(7), 1612.
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8

Microscopic Imaging of Mitotic and Meiotic Chromosomes

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Mitotic chromosomes were examined by Olympus BX53 epifluorescence microscope and Axio Imager Z2 microscope equipped with CCD camera (DP30W Olympus) and CoolCube 1 b/w digital camera (MetaSystems, Altlussheim, Germany), respectively. Axio Imager Z2 epifluorescence microscope is equipped by Metasystems platform for automatic search, capture and image processing. Meiotic chromosomes were analysed using Olympus BX63 microscopes equipped with standard fluorescence filter sets, and sCMOS camera (Prime95B Teledyne Photometrics) using CellSense Dimension software (Olympus). The IKAROS imaging program (Metasystems, Altlussheim, Germany) were used to analyse grey-scale images. The captured digital images from FISH experiments were pseudocoloured (blue for DAPI, red for Alexa-594, green for Alexa-488) and superimposed using Microimage and Adobe Photoshop software, version CS5, respectively. Microphotographs were finally adjusted and arranged in Adobe Photoshop CS6 software.
Marta A., Tichopád T., Bartoš O., Klíma J., Shah M.A., Bohlen V.Š., Bohlen J., Halačka K., Choleva L., Stöck M., Dedukh D, & Janko K. (2023). Genetic and karyotype divergence between parents affect clonality and sterility in hybrids. eLife, 12, RP88366.
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9

Automated Metaphase Chromosome Analysis

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The slides were stained with 5% Giemsa solution. Selected metaphases were captured using a Zeiss Axio Imager Z2 (Zeiss, Oberkochen, Germany), equipped with a Metafer-MSearch automatic scanning platform (MetaSystems, Altlussheim, Germany) and CoolCube 1 b/w digital camera (MetaSystems, Altlussheim, Germany). At least 20 metaphases per individual were analyzed. Karyograms were constructed using Ikaros karyotyping software (MetaSystems, Altlussheim, Germany).
Clemente L., Mazzoleni S., Pensabene E., Protiva T., Wagner P., Fritz U., Kratochvíl L, & Rovatsos M. (2021). Cytogenetic Analysis of the Asian Box Turtles of the Genus Cuora (Testudines, Geoemydidae). Genes, 12(2), 156.
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10

Automated Karyotyping and FISH Imaging

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Zeiss Axio Imager Z2, equipped with automatic Metafer-MSearch scanning platform (MetaSystems) and a CoolCube 1 b/w digital camera (MetaSystems, Altlussheim, Germany) was used to capture Giemsa-stained metaphases. Karyograms were prepared with software Ikaros (MetaSystems). A Provis AX70 (Olympus, Tokyo, Japan) fluorescence microscope equipped with a DP30BW digital camera (Olympus) was used to capture images from the FISH experiments, which were subsequently processed in DP MANAGER imaging software (Olympus).
Augstenová B., Mazzoleni S., Kostmann A., Altmanová M., Frynta D., Kratochvíl L, & Rovatsos M. (2019). Cytogenetic Analysis Did Not Reveal Differentiated Sex Chromosomes in Ten Species of Boas and Pythons (Reptilia: Serpentes). Genes, 10(11), 934.
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