Ginsenoside Rb1 (purity >98%) was obtained from a commercial source (Nanjing Spring & Autumn Biological Engineering Co., Ltd., Nanjing, China). Rotenone (R8875), dimethyl succinate (V900547), oligomycin (495455), and MitoB (SML1300) were purchased from Sigma (St. Louis, MO, USA). d15-MitoB (17470) and d15-MitoP (19296) were purchased from Cayman Chemical (Ann Arbor, MI, USA). Antibodies including anti-Ndufv1 (11238-1-AP), anti-Ndufv2 (15301-1-AP), anti-Ndufs1 (12444-1-AP), anti-Ndufs4 (15849-1-AP), anti-Ndufs6 (14417-1-AP), anti-Ndufa12 (15793-1-AP), anti-Bax (60267-1-Ig), and anti-Bcl2 (26593-1-AP) were purchased from ProteinTech (Chicago, IL, USA). Anti-prohibitin (ab75766) and anti-8 hydroxyguanosine (ab62623) antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-beta-actin (bs-0061R) antibody was purchased from Bioss (Beijing, China). Antibodies such as anti-Bak (BS1029), anti-histone H3 (BS1174), anti-GAPDH (AP0063), goat anti-rabbit IgG (H+L) HRP (BS13278), and goat anti-mouse IgG (H+L) HRP (BS12478) were purchased from Bioworld Technology (St. Paul, MN, USA).
Goat anti mouse igg h l hrp bs12478
Manufactured by Bioworld Technology
Sourced in United States
Goat anti-mouse IgG (H+L) HRP (BS12478) is a secondary antibody reagent that can be used for the detection of mouse immunoglobulins (IgG) in various immunoassays. It is conjugated with the enzyme horseradish peroxidase (HRP), which enables colorimetric or chemiluminescent detection.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit igg h l hrp bs13278, by Bioworld Technology (5 mentions)
Anti bcl2 26593 1 ap, by Proteintech (1 mentions)
Ecl western blotting substrate kit, by Merck Group (1 mentions)
Anti rabbit igg or antimouse igg, by Beyotime (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
P pi3k, by Cell Signaling Technology (1 mentions)
Ab70358, by Abcam (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Gapdh ap0063, by Bioworld Technology (1 mentions)
G 6 pase sc 15840, by Santa Cruz Biotechnology (1 mentions)
Acetylated lysine 9441, by Cell Signaling Technology (1 mentions)
Tissue mitochondria isolation kit, by Beyotime (1 mentions)
Calcium ionophore a23187, by Merck Group (1 mentions)
Actin 66009 1 ig, by Proteintech (1 mentions)
Cocl2, by MedChemExpress (1 mentions)
Fluo 4am s1060, by Beyotime (1 mentions)
P62 18420 1 ap, by Proteintech (1 mentions)
H3k4me3 ab1012, by Abcam (1 mentions)
5 protocols using goat anti mouse igg h l hrp bs12478
1
Mitochondrial Dysfunction Analysis Reagents
2
Protein Expression Analysis in Frozen Tissues
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The frozen tissues were ground into powder with an ultrasound after adding radio immunoprecipitation assay (RIPA) lysis buffer containing protease and phosphatase inhibitors. The samples were centrifuged for 5 minutes at 12,000 ×g, and the supernatant was collected for protein quantitation by a bicinchoninic acid (BCA) assay. Equal volumes (50 mg) of protein were separated using SDS-PAGE and transferred to nitrocellulose membranes. The membranes were incubated overnight at 4°C in 5% skim milk with the following primary antibodies: p-PI3K, p-AKT (Ser473), p-mTOR (all 1 : 1000, Cell Signaling Technology, USA), phospho-p62, LC3-II (all 1 : 1000, Affinity, USA), Nox2 (1 : 1000, ab31092, USA), and GAPDH (1 : 1000, Cell Signaling Technology, USA). The membranes were then washed and incubated using a secondary anti-rabbit IgG or anti-mouse IgG (all 1 : 10000, Beyotime Institute of Biotechnology, China). The corresponding secondary antibodies goat anti-mouse IgG (H+L)-HRP (BS12478) and goat anti-rabbit IgG (H+L)-HRP (BS13278) were all purchased from Bioworld Technology Company, and all the dilution for Western blotting is 1 : 5000. Band visualization was performed using an ECL Western Blotting Substrate kit (Millipore, 1622301, USA).
3
Mitochondrial Acetylome Analysis in Liver
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Hepatocytes or liver tissue were lysed with ice-cold RIPA buffer and the protein extraction were immunoblotted to analysis the protein expression. The protein lysates were separated by SDS-PAGE and transferred onto polyvinylidene fluoride membranes (Millipore Co, Ltd.). Immunoblotting was performed according to standard procedures with the following primary antibodies: G-6-Pase (sc-15840, 1:500) and SIRT3 (sc-365175, 1:500) antibodies were from Santa Cruz Biotechnology, Inc. (Santa, CA, USA), antibodies for PEPCK (ab70358, 1:1000), PC (ab128952, 1:1000), p-PDH (S293) (ab177461, 1:1000) were from Abcam (Cambridge, MA, USA), antibodies of MPC1 (14462, 1:1000), PDH (2784, 1:1000) and Acetylated-Lysine (9441, 1:1000) were obtained from Cell Signaling Technology (Beverly, MA, USA), VDAC1 (CY5416, 1:1000) antibody was from Abways Technology, Inc. and antibodies of GAPDH (AP0063, 1:3000), Goat Anti-Rabbit IgG (H + L) HRP (BS13278, 1:10000) and Goat Anti-Mouse IgG (H + L) HRP (BS12478, 1:10000) were from Bioworld Technology (St. Paul, MN, USA). To determine the mitochondrial acetylated-lysine in the liver, mitochondrial protein was prepared with Tissue Mitochondria Isolation Kit (Beyotime Institute of Biotechnology, Shanghai, China) as manufacturer's instructions.
4
CaMKII Signaling in Cellular Stress Response
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GAS (SMB00313), CoCl2 (232696), and calcium ionophore (A23187) were purchased from Sigma-Aldrich (St Louis, MO, USA). GAS and CoCl2 were dissolved in phosphate-buffered solution to prepare the original solution of 100 mM, which was stored at -20° C. CQ diphosphate (HY-17589) and 3-BDO (HY-U00434) were purchased from MedChemExpress. Fluo-4AM (S1060) was obtained from Beyotime Biotechnology, Inc. (Nanjing, China). Primary antibodies against β-actin (66009-1-Ig, 1:10 000), LC3 (14600-1-AP, 1:1000), p62 (18420-1-AP, 1:1000), p62(66184-1-lg), LAMP-2 (66301-1-lg, 1:1000), and CaMKIIα (11533-1-AP, 66843-1-Ig) were purchased from Proteintech (Wuhan, China). Rabbit anti-p-p62 (Thr349) (Ab211324) was purchased from Abcam (Cambridge, MA, USA). Primary antibodies against CaMKIIα (#4436, 1:1000) and anti-p-CaMKIIα (Thr286) (#12716, 1:1000) were obtained from Cell Signaling Technology (Danvers, MA, USA). Goat anti-rabbit IgG (H&L)-HRP (BS13278, 1:10000) and goat anti-mouse IgG (H&L)-HRP (BS12478) were obtained from Bioworld. Goat PAb to Rb IgG Alexa Fluor®488 (Ab150077, 1:200) and goat PAb to MS IgG Alexa Fluor®647 (Ab150115) were purchased from Abcam. A fluorescein isothiocyanate-conjugated goat anti-rabbit secondary antibody (A0516, 1:200) was purchased from Beyotime Biotechnology.
5
Antibody Characterization and Validation
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Znhit1 (ab238125), H2A.Z (ab4174), and H3K4me3 (ab1012) were purchased from Abcam (Cambridge); H3K27me3 (9733) was from Cell Signaling Technology. Casq1 (26665-1-AP), Casq2 (18422-1-AP), ATP2A2/SERCA2a (26665-1-AP), for Western blotting, were purchased from Proteintech; ATP2A2/SERCA2a (sc-376235), for IF, was purchased from Santa Cruz; Gapdh Ab (AP0063), goat anti–mouse IgG (H+L)-HRP (BS12478), goat anti–rabbit IgG (H+L)-HRP (BS13278) were purchased from Bioworld; Alexa Fluor 488/Cy3/Cy5–labeled secondary Abs (115-514-166,112-165-167, and 711-175-152) were purchased from Jackson Immunoresearch.
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