Spectrum one ftir

The pristine fiber, fiber blend, and Plai oil-loaded fiber blend membranes were analyzed by a Fourier-transform infrared spectrometer (Perkin-Elmer Spectrum One FTIR, USA). The spectra were collected from 4000 cm⁻¹ to 650 cm⁻¹ with 64 scans and a resolution of 4 cm⁻¹.

The instrument that was used for the acquisition of the IR absorption spectra of the cell samples was a Spectrum One FTIR (PerkinElmer, Shelton, CT, USA) spectrometer, equipped with a Perkin Elmer Multiscope system infrared microscope and an MCT (mercury cadmium telluride) detector. The measurements were performed on cells adherent on MirrIR slides having an area of 25 × 25 mm² in transflection mode. The background signal was acquired in a region of the MirrIR slide that was free of cells. For each experimental condition, three slides were prepared. Every slide was examined in different regions of about 100 × 100 μm size (see Figure 4), and three spectra were acquired for each position. The measurements were made at room temperature by collecting the signal in the spectral region between 4000 and 800 cm⁻¹ using 64 scans with a spectral resolution of 4 cm⁻¹ and a 5 s acquisition time for each spectrum.

Unless otherwise stated, all
irradiations were performed using a 15 W, Analytik Jena UV bench lamp
fitted with 254 nm (UVC) bulbs which provided an incident irradiance
of 2 mW cm^–2. The irradiance was measured using
a UVX meter (Analytik Jena, Jena, Germany) fitted with a 254 nm sensor.
All FTIR spectra were recorded using a Spectrum One FTIR (PerkinElmer,
Massachusetts, USA). Digital photographs were taken using a Canon
77D fitted with a Canon EF-S 60 mm f2.8 USM Macro Lens. Optical microscopy
was carried out using an Olympus Trinocular Microscope, SZ6045TR (Tokyo,
Japan), fitted with a Kiralux 8.9 MP color CMOS camera, CS895CU (ThorLabs,
New Jersey, USA). Profilometry measurements were carried out using
a Dektak3ST surface profile measuring system (Veeco, California, USA).
The SA dots were scanned over a distance of 5 mm at a rate of 100
μm s^–1, with a stylus force of 3 mg.
Irradiations of the SA volcano islands were performed inside the
profilometer. In this work, UVA light (365 nm, ca. 80 mW cm^–2) was supplied by a 10 W 365 nm LED, ILH-XT01-S365-SC211-WIR200 (Intelligent
LED Solutions, Berkshire, UK), through an optical fiber, BFY1000HS02
(ThorLabs, New Jersey, USA), held ca. 2–3 mm above the surface
of the SA volcano island. The irradiance was measured using a C10427
UV power meter (Hamamatsu, Shizuoka, Japan).

The conversion of the resulting membranes to TR-PBO membranes was monitored via attenuated total reflectance-Fourier transform infrared (ATR-FTIR) using a PerkinElmer Spectrum One FT-IR (PerkinElmer, Waltham, MA, USA) coupled with a universal attenuated total reflection (ATR) diamond-tipped sampling module following the band’s intensity.

Spectra were collected to analyse and compare the material characteristics of all scaffold samples, specifically CH only, CH:HA bicomposite and CH:HA:FU tricomposite. The FTIR analysis was carried out using a Spectrum One FTIR (Perkin Elmer, Beaconsfield, UK). The scaffolds were finely cut and mixed with potassium bromide before being pressed and analysed. The FTIR spectra were collected with wavenumbers between 4000 and 400 cm⁻¹. The scans were evaluated to determine the major elements present and to detect the presence of HA and FU in the bi- and tricomposite scaffolds.

Fourier-transform infrared spectroscopy was measured on a PerkinElmer Spectrum One FTIR. The freeze-dried samples were milled to powder and mixed with KBr. The mixed powder were pressed to pieces. The analysis was operated by scanning scale of 4000–400 cm⁻¹ for 32 times.

To study the optical properties of light filters, optical transmittance studies were conducted for the ultraviolet, visible, and infrared regions of the spectrum, using round and rectangular samples.
Optical transmission tests of light filter samples were performed on:-

Optizen 3220 UV (Mecasys Co., Ltd, Daejeon, Korea) double-beam spectrophotometer, which is designed for measuring transmission coefficients, optical density and scanning transmission or absorption spectra in a given wavelength range of ultraviolet and visible radiation from 190 nm to 1100 nm;

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Spectrometers Spectrum One FT-IR (PerkinElmer Inc., Waltham, MA, USA) in the wavelength range from 1.25 μm to 10 μm.

Measurements were performed with a scanning step of 1 nm.