HeLa cells were cultured in Dulbecco´s modified Eagle´s medium supplemented with 10% fetal bovine serum in a 5% CO2 incubator. For RNA interference, Opti-MEM medium and Lipofectamine 3000 (ThermoFisher) was used as per the manufacture´s protocol. HeLa cells were transfected with control siRNA (non-targeting pool, Dharmacon) or siRNAs targeting human leucyl-tRNA synthetase 1 or 2 (Dharmacon), separately. For transfection of tRNA plasmids, HeLa cells were transfected by Lipofectamine 3000 with 2 μg of plasmid encoding tRNA wild-type or variant genes and incubated for 24 hours before RNA extraction.
Sirnas targeting human leucyl trna synthetase 1 or 2
Manufactured by Horizon Discovery
SiRNAs targeting human leucyl-tRNA synthetase 1 or 2 are small interfering RNAs designed to downregulate the expression of the corresponding genes. Leucyl-tRNA synthetase is an essential enzyme involved in protein synthesis.
Automatically generated - may contain errors
2 protocols using sirnas targeting human leucyl trna synthetase 1 or 2
1
RNA Interference and Plasmid Transfection in HeLa Cells
2
HeLa cells gene knockdown and overexpression
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HeLa cells were cultured in Dulbecco´s modified Eagle´s medium supplemented with 10% fetal bovine serum in a 5% CO 2 incubator. For RNA interference, Opti-MEM medium and Lipofectamine 3000 (ThermoFisher) was used as protocol. HeLa cells were transfected with control siRNA (nontargeting pool, Dharmacon) or siRNAs targeting human leucyl-tRNA synthetase 1 or 2 (Dharmacon) separately. For transfection of tRNA plasmid, HeLa cells were transfected by Lipofectamine 3000 with 2 μg of plasmid encoding tRNA wild-type or variant genes and incubated for 24 hours before RNA extraction.
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