MilliQ water purged with nitrogen to remove carbon dioxide and L- -amino acids were used throughout this work. Di-sodium succinate, sodium aspartate monohydrate, sodium glutamate monohydrate, aluminum chloride hexahydrate (AlCl3 · 6H2O; ≥99.0%), magnesium chloride (MgCl2 · 6 H2O; ≥99.0%), and sodium carbonate (Na2CO3; ≥99.5%) were purchased from Sigma-Aldrich. Perchloric acid (HClO4; 60%) was purchased from Riedel-de haën. Sodium hydroxide (NaOH; 1M) was purchased from Corto Erba reagents groups. All products were used as received without any further treatment.
Perchloric acid
Manufactured by Honeywell
Sourced in Germany
Perchloric acid is a highly corrosive and oxidizing chemical compound with the formula HClO4. It is a strong acid commonly used in analytical and industrial chemistry applications.
Automatically generated - may contain errors
Lab products found in correlation
Nitric acid, by Honeywell (2 mentions)
Disodium succinate, by Merck Group (1 mentions)
Aluminum chloride hexahydrate, by Merck Group (1 mentions)
Magnesium chloride, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Trichloroacetic acid, by Honeywell (1 mentions)
Methyl isobutyl ketone mibk, by Merck Group (1 mentions)
Tryptic soy agar, by BD (1 mentions)
Ammonium heptamolybdate tetrahydrate, by Merck Group (1 mentions)
Cholesterol from ovine wool, by Avanti Polar Lipids (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
5 protocols using perchloric acid
1
Amino Acid-based Chemical Synthesis
2
Determination of Lead in Solutions
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Analytical standard solutions of lead (Spectro ECON) were prepared by serially diluting a 1000 mg/L stock calibration standard solution. Reagents used included trichloroacetic acid (5%; TCA) and perchloric acid (extra pure, Riedel-de Haen, Germany), K2EDTA and ammonium pyrollidine dithiocarbamates (APDC; Merck (Darmstadt), calcium chloride and methyl iso-butyl ketone (MIBK; Sigma Chemical Co).
3
Herbal Sample Preparation for Analysis
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The following reagents were used throughout this study: tryptic soy agar (Difco Laboratories, Detroit, USA), Sabouraud dextrose agar (Difco™; France) (for preparing the medium), tryptic soy broth (Difco™; France), and Tween 80 (Difco™; France), which was used to prepare Fluid 3 for microbiological testing. Nitric acid (70%) (Riedel-de Haen, Seelze, Germany) and perchloric acid (70%–72%) (Riedel-de Haen, Seelze, Germany) were used for acid digestion of the herbal samples.
4
Physicochemical Characterization of Drug-Lipid Interactions
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Diclofenac sodium salt (DIC), naproxen sodium salt (NAP), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), Trizma® base, sodium chloride, sodium hydroxide, potassium phosphate monobasic, ascorbic acid, 1,3,6,8-pyrenetetrasulfonic acid tetrasodium salt hydrate (PTS), methyl viologen dichloride hydrate (MV), Sephadex G-25® medium, and nonaethylene glycol monododecyl ether (polidocanol) were supplied by Sigma-Aldrich Co. (St. Louis, MO, USA). Cholesterol from ovine wool (CHOL), 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-glycerol (sodium salt) (TMCL), cardiolipin sodium salt from bovine hearth (CL), and L-α-phosphatidylinositol sodium salt from bovine liver (PI) was supplied by Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Chloroform and methanol were obtained from VWR International S.A.S. (Fontenais-sous-Bois, France). Ammonium heptamolybdate tetrahydrate and hydrochloric acid were acquired from E. Merck (Darmstadt, Germany) and Fisher Scientific UK (Loughborough, UK), respectively. Perchloric acid was purchased by Riedel-de Haen (Seelze, Germany).
All assays were performed mimicking physiological conditions using Tris-HCl buffer (pH 7.4). It was prepared by dissolving Trizma® base (10 mM) and NaCl (150 mM) in double deionized water (κ < 0.1 μS.cm−1) and adjusting the pH value using an HCl solution (1 M).
All assays were performed mimicking physiological conditions using Tris-HCl buffer (pH 7.4). It was prepared by dissolving Trizma® base (10 mM) and NaCl (150 mM) in double deionized water (κ < 0.1 μS.cm−1) and adjusting the pH value using an HCl solution (1 M).
5
Acid Digestion and Microbial Media Preparation
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All the reagents used were purchased from reliable resources, the following reagents were used throughout the research project: Nitric Acid 70% (Riedel-de-Haen™; Germany), Perchloric Acid 70% – 72% (Riedel-de-Haen™; Germany) were used in acid digestion of herbals, Tryptic Soy Agar ( Difco ™; France), Sabouraud Dextrose Agar (Difco™; France) were used in media preparation, Tryptic Soy Broth (Difco™; France), Tween80 (Difco™; France) were used in Fluid 3 preparation used in the microbiological testing.
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