A fluorescein-conjugated Annexin V (Annexin V-FITC) staining assay kit (BD Biosciences, San Jose, CA, USA) was used to quantitatively assess the level of induced cell apoptosis. Briefly, the treated cells were washed with PBS, stained with 5 μL Annexin V-FITC and 5 μL, and incubated for 15 min at room temperature in the dark. The degree of apoptosis was then quantified as the percentage of Annexin V-positive and PI-negative (Annexin V+/PI−) cells using a flow cytometer.
Fluorescein conjugated annexin 5 annexin 5 fitc staining assay kit
Manufactured by BD
Sourced in United States
The Fluorescein-conjugated Annexin V (Annexin V-FITC) staining assay kit is a laboratory tool used to detect and quantify apoptosis, a programmed cell death process. The kit contains Annexin V, a protein that binds to phosphatidylserine, a molecule exposed on the surface of apoptotic cells. Annexin V is conjugated with the fluorescent dye FITC, allowing the identification of apoptotic cells through fluorescence microscopy or flow cytometry.
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2 protocols using fluorescein conjugated annexin 5 annexin 5 fitc staining assay kit
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Annexin V-FITC Apoptosis Assay
2
Apoptosis Detection by Annexin-V/PI Staining
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A combination of annexin-V and propidium iodide (PI) staining is used to distinguish between early (annexin-V + / PI -) and late (annexin-V + /PI -) apoptotic, necrotic, and live cells using a fluorescein-conjugated annexin V (annexin V-FITC) staining assay kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer's protocol (BD Biosciences). Briefly, cells were washed with phosphate buffered saline and resuspended in 100 μl binding buffer containing 5 μl annexin-V-FITC and 5 μl PI for 15 min at room temperature in the dark. The cells were immediately analyzed by flow cytometry (FACS Calibur, Becton Dickinson, San Jose, CA, USA). The percentages of apoptotic cells (annexin-V + cells) were presented as the mean ± standard deviation (SD) as described previously (Kim et al. 2014) (link).
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