The MUC1, EpCAM, BSA, PSA and VEGF were purchased from Cusabio Biotech Co. Ltd. (http://www.cusabio.cn/ ). Graphene oxide was purchased from XFNANO Co. Ltd. (Nanjing, China, http://www.xfnano.com ). The deoxyribonuclease I (DNase I) were obtained from solarbio (Beijing, China, http://www.solarbio.com ). The MUC1 (5′-FAM-CCCGTCTTCCAGACAAGAGTGCAGGG-3′) aptamer were synthesized by Sangon Biological Engineering Technology Co., Ltd. (Shanghai, China, http://www.sangon.com ) and purified using high performance liquid chromatography. All of the reagents were diluted to the required concentration with working buffer (10 mM Tris, 100 mM NaNO3 pH 7.4) before usage. Healthy human serum, urine and saliva were provided by Affiliated Dongfeng Hospital, Hubei University of Medicine, and approved by Hospital's Ethics Committee. The other reagents employed were of analytical grade and used without further purification. Ultrapure water obtained from a Millipore water purification system (18.2 MΩ cm resistivity, Milli-Q Direct 8) was used in all runs.
Bovine serum albumin (bsa)
Manufactured by Cusabio
BSA (Bovine Serum Albumin) is a laboratory reagent used as a protein standard in various biochemical and analytical applications. It is a purified form of the albumin protein derived from bovine (cattle) serum. BSA is commonly used to quantify protein concentrations, block non-specific binding sites, and stabilize enzymes and other proteins in biological assays.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using bovine serum albumin (bsa)
1
Multiplexed Biosensor for Cancer Biomarkers
2
Quantitative Detection of Biomarkers
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The deoxyribonuclease I (DNase I) were obtained from solarbio (Beijing, China, http://www.solarbio.com ). The MB, CD63, BSA, EPCAM and VEGF were purchased from Cusabio Biotech Co. Ltd. (http://www.cusabio.cn/ ). The MB aptamer (5′-CCCTCCTTTCCTTCGACGTAGATCTGCTGCGTTGTTCCGA-3′-FAM) were synthesized by Sangon Biological Engineering Technology Co., Ltd. (Shanghai, China, http://www.sangon.com ) and purified using high performance liquid chromatography. All reagents employed were of analytical grade and used without further purification. And were diluted to the required concentration with working buffer (10 mM Tris, 100 mM NaNO3, pH 7.4) before usage. This study all experiments were performed in compliance with the relevant laws and institutional guidelines (National Health Commission of the people's Republic of china, ethical review of biomedical research involving human beings), healthy human serum, urine and saliva were provided by Affiliated Dongfeng Hospital, Hubei University of Medicine, and approved by Dongfeng Hospital's Ethics Committee, informed consent was obtained for any experimentation with human subjects. Ultrapure water obtained from a Millipore water purification system (18.2 MΩ cm resistivity, Milli-Q Direct 8) was used in all runs.
3
Biosensing Molecular Interactions Protocol
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The EpCAM, PSA, BSA and VEGF165 were purchased from Cusabio Biotech Co. Ltd. All of the reagents were diluted to the required concentration with working buffer (20 mM Tris–HCl, 100 mM NaCl, pH 7.4) before use. All of the oligonucleotides used in this work were synthesized and purified using HPLC by Sangon Biotechnology Co. Ltd. (Shanghai, China, https://www.sangon.com ), and their sequences are shown in Table S1.† GO was purchased from XFNANO Co. Ltd. (Nanjing, China, https://www.xfnano.com ). The other reagents employed were of analytical grade and used without further purification. Healthy human serum, urine and saliva were provided by Affiliated Dongfeng Hospital, Hubei University of Medicine, and approved by the Hospital's Ethics Committee. Ultrapure water obtained from a Millipore water purification system (18.2 MΩ cm resistivity, Milli-Q Direct 8) was used in all runs.
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