Mouse anti atp5b

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Mouse anti-ATP5B antibody is a research-use-only product designed to detect the ATP5B protein, which is a subunit of the ATP synthase complex found in the mitochondria. This antibody can be used for applications such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of the ATP5B protein in various biological samples.

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Lab products found in correlation

Alexa fluor 594, by Thermo Fisher Scientific (2 mentions) Alexa fluor 647, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Mitotracker cmxros, by Thermo Fisher Scientific (1 mentions) Rabbit anti tomm20, by Santa Cruz Biotechnology (1 mentions) Antimycin a, by Santa Cruz Biotechnology (1 mentions) Oligomycin, by Santa Cruz Biotechnology (1 mentions) Nb100 2220, by Novus Biologicals (1 mentions) Mouse anti parkin, by Cell Signaling Technology (1 mentions) Gp62 c, by Novus Biologicals (1 mentions) Irdye 800 goat anti mouse igg, by LI COR (1 mentions) Iblot dry blotting system, by Thermo Fisher Scientific (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Irdye 700 goat anti rabbit igg, by LI COR (1 mentions) Triton x 100, by Thermo Fisher Scientific (1 mentions) Goat or donkey serum, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) S5545, by Abcam (1 mentions)

Close spelling variants of this product

Anti-ATP5B (8 citations) ATP5B (7 citations)

4 protocols using mouse anti atp5b

Mitochondrial Dysfunction and Autophagy Analysis

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Antimycin A (Santa Cruz), oligomycin (Santa Cruz), CCCP (Sigma), bafilomycin A1 (Sigma), and MitoTracker CMXROS (ThermoFisher) were resuspended in DMSO (Sigma). Antimycin A and oligomycin were stored in aliquots at −80°C. CCCP and bafilomycin A1 were stored in aliquots at −20°C. Primary antibodies for immunofluorescent staining include the following: rabbit anti‐LC3 (Sigma L7543, 1:5,000), rabbit anti‐TOMM20 (Santa Cruz sc‐11415, 1:1,000), mouse anti‐Parkin (Cell Signaling 4211, 1:1,000), mouse anti‐DNA (Millipore CBL186, 1:1,000), mouse anti‐ATP5B (Santa Cruz sc‐166462, 1:1,000), mouse anti‐PEX13 (Santa Cruz sc‐271477, 1:100), rabbit anti‐PMP70 (Thermo Scientific PA1‐650, 1:1,000), mouse anti‐Flag (Sigma 184‐200UG, 1:1,000), and rabbit anti‐WIPI2 (Abcam ab105459, 1:500). Secondary antibodies were conjugated to AlexaFluor488, AlexaFluor594, and/or AlexaFluor647 (Invitrogen, 1:750). Primary antibodies for Western blot analyses include the following: mouse anti‐PEX13 (Santa Cruz sc‐271477, 1:200), rabbit anti‐ATG7 (Sigma A2856, 1:1,000), rabbit anti‐FANCC (Fanconi Anemia Research Fund FANCC‐C2, 1:1,000), mouse anti‐SMURF1 (Sigma WH0057154M1, 1:1,000), guinea pig anti‐p62 (Progen GP62‐C, 1:1,000), rabbit anti‐LC3 (Novus NB100‐2220, 1:1,000), and HRP‐conjugated mouse anti‐actin (Santa Cruz sc‐47778‐HRP, 1:2,000).

Lee M.Y., Sumpter R J.r., Zou Z., Sirasanagandla S., Wei Y., Mishra P., Rosewich H., Crane D.I, & Levine B. (2016). Peroxisomal protein PEX13 functions in selective autophagy. EMBO Reports, 18(1), 48-60.

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Quantitative Protein Analysis by Immunoblotting

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For immunoblotting (10 μg), proteins were electrotransferred onto nitrocellulose membranes using the iBlot™ Dry Blotting System (Life Technologies) and membranes blocked with 5% (w/v) skim milk powder in Tris-buffered saline with 0.05% (v/v) Tween-20 (TTBS) for 1 h. Membranes were probed with primary antibodies [mouse anti-ATP5B (Santa Cruz Biotechnology; 1:200), mouse anti-GPD2 (Santa Cruz Biotechnology; 1:200), rabbit anti-ARF4 (Abcam; 1:1000), rabbit anti-SDC2/HSPG2 (OriGene; 1:500)] for 10 h in TTBS (50 mM Tris, pH 7, 150 mM NaCl, 0.05% (v/v Tween 20) at 4°C, followed by incubation with either IRDye 800 goat anti-mouse IgG or IRDye 700 goat anti-rabbit IgG (1:15000, LI-COR Biosciences). Fluorescent signals were detected using the Odyssey Infrared Imaging System, (v3.0, LI-COR Biosciences, Nebraska USA).

Greening D.W., Lee S.T., Ji H., Simpson R.J., Rigopoulos A., Murone C., Fang C., Gong S., O'Keefe G, & Scott A.M. (2015). Molecular profiling of cetuximab and bevacizumab treatment of colorectal tumours reveals perturbations in metabolic and hypoxic response pathways. Oncotarget, 6(35), 38166-38180.

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Immunofluorescence Imaging of Serotonin and Mitochondria

Cited 1 time

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Frozen mouse brain sections were prepared according to previous protocol [42 (link)]. Mouse brains were dissected and fixed in 4% paraformaldehyde (Sigma-Aldrich, #158127) overnight at 4°C. Primary cultured raphe neurons were fixed in 4% paraformaldehyde for 30 min at 37°C. Brain slices or cultured raphe neurons were treated with blocking buffer (5% goat or donkey serum (Sigma-Aldrich), 0.3% Triton X-100 (Fisher Scientific) in PBS, pH 7.4) and probed with primary antibodies accordingly: rabbit-anti-serotonin (Sigma-Aldrich, #S5545, 1:400), rabbit-anti-serotonin transporter (abcam, #ab25358, 1:400), mouse-anti-ATP5B (Santa Cruz, #sc-55597, 1:500). After overnight incubation with primary antibodies, the brain slices or neurons were probed with proper cross-adsorbed secondary antibodies conjugated to Alexa Fluor 488, Alexa Fluor 594, or Alexa Fluor 647 (Thermo Fisher Scientific, 1:500). Cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI). Images were collected on a Nikon confocal microscope. Serotonin intensity, serotonergic fiber density, and mitochondrial volume and density were analyzed using Nikon-Elements Advanced Research software. Mitochondrial density was calculated as number of mitochondria per μm serotonergic fiber.

Sheldon C.C., Rouse D.T., Finnegan E.J., Peacock W.J, & Dennis E.S. (2000). The molecular basis of vernalization: The central role of FLOWERING LOCUS C (FLC). Proceedings of the National Academy of Sciences of the United States of America, 97(7), 3753-3758.

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In Vivo Study of H3K4me3 and H3K36me3 in Cancer

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Institute of Cancer Research (ICR) mice were used in this study. All experiments were approved by the Animal Ethics Committee of the Third Affiliated Hospital of Guangzhou Medical University and were performed in accordance with the guidelines of Animal Care and Use of the Third Affiliated Hospital of Guangzhou Medical University (TAHGMUACC-2019-0126).
Unless otherwise specified, all chemicals and reagents used in this study were purchased from Sigma Chemical Company (St. Louis, MO, USA). OBZ was purchased from Selleck Chemicals (Houston, TX, USA). A rabbit anti-H3K4me3 antibody and a rabbit anti-H3K36me3 antibody were purchased from Cell Signaling Technology (Devers, MA, USA). Mouse anti-ATP5A and mouse anti-ATP5B antibodies were purchased from Santa Cruz (Santa Cruz, CA, USA). Alexa Fluor 488 goat anti-rabbit and Alexa Fluor 594 goat anti-mouse antibodies were purchased from Invitrogen (Carlsbad, CA, USA).

Jin L., Zhu H.Y., Kang X.J., Lin L.P., Zhang P.Y., Tan T., Yu Y, & Fan Y. (2020). Melatonin protects against oxybenzone-induced deterioration of mouse oocytes during maturation. Aging (Albany NY), 13(2), 2727-2749.

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