Log In Sign up
The largest database of trusted experimental protocols

Anti ccnd1

Manufactured by Proteintech
Visit Supplier
Sourced in United States

Anti-CCND1 is a laboratory reagent used for the detection and quantification of the CCND1 (Cyclin D1) protein in various biological samples. It is a primary antibody that specifically binds to the CCND1 protein, which is a key regulator of the cell cycle. The core function of Anti-CCND1 is to enable researchers to study the expression and localization of the CCND1 protein in their experiments.

Automatically generated - may contain errors

Lab products found in correlation

Anti cdk4, by Proteintech (4 mentions) Anti gapdh, by Proteintech (3 mentions) Anti e cadherin, by Proteintech (3 mentions) Anti mmp2, by Proteintech (3 mentions) Pvdf membrane, by Merck Group (2 mentions) Anti fam84b, by Proteintech (2 mentions) Anti npm1, by Proteintech (2 mentions) Anti e2f1, by Proteintech (2 mentions) Anti mmp9, by Proteintech (2 mentions) Anti vimentin, by Proteintech (2 mentions) Anti cdk6, by Proteintech (2 mentions) Anti dnmt3b, by Cell Signaling Technology (2 mentions) Anti nop14, by Proteintech (2 mentions) Anti ki67, by Proteintech (2 mentions) Bca assay kit, by Boster Bio (1 mentions) Anti cdkn2a, by Proteintech (1 mentions) Anti ha, by Abcam (1 mentions) Anti gst, by Proteintech (1 mentions) Anti flag, by Cell Signaling Technology (1 mentions) Anti prb, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

CCND1 (11 citations) Anti-TM (2 citations) Anti-CCND1 mouse monoclonal antibody (2 citations) Mouse anti-CCND1 (2 citations)

6 protocols using anti ccnd1

1

Western Blot Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein levels of the genes were detected through western blot. Briefly, cells were lysed using RIPA buffer for 1 h on ice, the protein concentrations were determined via a BCA assay kit (Boster, Wuhan). The proteins were separated by SDS-PAGE. 50 μg of protein and 4×loading buffer were boiled for 10 min and separated by SDS-PAGE (10% separating gel and 5% stacking gel), the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA) that were subjected to blocking by 5% skimmed milk for 1 h at room temperature, then incubated with the specific antibodies at 4 °C overnight. The goat anti-mouse and goat anti-rabbit second antibodies were got from Odyssey. The relative amount of gene product was normalized to GAPDH levels.
Proteins were detected by using anti-FAM84B (Proteintech, 18421-1-AP), anti-NPM1 (Proteintech, 60096-1-Ig), anti-CCND1 (Proteintech, 60186-1-Ig), anti-CDK4 (Proteintech, 11026-1-AP), CDK6 (Proteintech, 14052-1-AP), anti-FLAG (Cell Signaling, #14793), anti-HA (Abcam, 9110), anti-GST (Proteintech, 66001-2-Ig), anti-pRb (Cell Signaling, #8516), anti-CDKN2A (10883-1-AP), anti-E2F1 (12171-1-AP) and anti-GAPDH (Proteintech, 60004-1-Ig).
Wang F., Cheng C., Wang X., Chen F., Li H., Zhou Y., Wang Y., Hu X., Kong P., Zhang L., Cheng X, & Cui Y. (2022). Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma. Cell Death Discovery, 8, 182.
+ Open protocol
+ Expand
2

TRAF4 Regulation in Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this article include anti-TRAF4 (Proteintech: Wuhan, China, CAS: 66755-1-Ig), anti-TRAF4 (Abcam: NY, USA, CAS: ab245666), anti-Tubulin (Proteintech: Wuhan, China, CAS: 66031-1-Ig), anti-E-cadherin (Proteintech: Wuhan, China, CAS: 20874-1-AP), anti-MMP2 (Proteintech: Wuhan, China, CAS: 10373-2-AP), anti-β-catenin (Cell Signaling Technology: Danvers, MA, USA, CAS: 8480), anti-CCND1 (Proteintech: Wuhan, China, CAS: 60186-1-Ig), anti-GSK3β (Proteintech: Wuhan, China, CAS: 22104-1-AP), anti-p-GSK3β (Cell Signaling Technology: Danvers, MA, USA, CAS: 5558), anti-AKT1 (Proteintech: Wuhan, China, CAS: 60203-2-Ig), anti-p-AKT (Cell Signaling Technology: Danvers, MA, USA, CAS: 13038), anti-SETDB1(Cell Signaling Technology: Danvers, MA, USA, CAS: 2196), anti-Flag (Proteintech: Wuhan, China, CAS: 66008-3-Ig), anti-Flag (Proteintech: Wuhan, China, CAS: 80010-1-RR), anti-MYC-tag (Proteintech: Wuhan, China, CAS: 60003-2-Ig), anti-MYC-tag (Proteintech: Wuhan, China, CAS: 16286-1-AP), and anti-HA (Proteintech: Wuhan, China, CAS: 51064-2-AP).
Reagents used in this article including Cycloheximide (CHX) (Merck: Kenilworth, NJ, USA, CAS: 66-81-9) and proteasome inhibitor (MG-132) (MCE: Chongqing, China, CAS: 133407-82-6).
Gu H., Zhu S., Peng C., Wei Z., Shen Y., Yuan C., Yang H., Cui H, & Yang L. (2022). TRAF4 Promotes the Proliferation of Glioblastoma by Stabilizing SETDB1 to Activate the AKT Pathway. International Journal of Molecular Sciences, 23(17), 10161.
+ Open protocol
+ Expand
3

Western Blot Antibody Screening Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot assays were conducted as previously described.8 The associated primary immunoblotting antibodies were as follows: anti‐GAPDH, anti–E‐cadherin, anti–N‐cadherin, anti‐Vimentin, anti‐CDK6, anti‐CCND1, anti‐CDK4, anti‐NOP14, anti‐MMP9, anti‐MMP2, anti‐E2F1, anti‐MET, anti‐Parp‐1, (Proteintech Group), anti‐Caspase 3, anti‐DNMT3B (Cell Signaling Technology) and anti‐SP1 (Santa Cruz Biotechnology).
Ying Y., Li J., Xie H., Yan H., Jin K., He L., Ma X., Wu J., Xu X., Fang J., Wang X., Zheng X., Liu B, & Xie L. (2020). CCND1, NOP14 and DNMT3B are involved in miR‐502‐5p–mediated inhibition of cell migration and proliferation in bladder cancer. Cell Proliferation, 53(2), e12751.
+ Open protocol
+ Expand
4

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total proteins were extracted by lysing cells in 6‐well plate with 60 μL RIPA buffer supplemented with protease inhibitors (#G2006, Wuhan Goodbio Technology) and phosphatase inhibitors (#G2007, Wuhan Goodbio Technology) and quantified by BCA protein assay kit (#P0011, Beyotime). One microgram of proteins was separated by 10% SDS‐PAGE and transferred to PVDF membrane (#IPVH00010, Merck Millipore). The membrane was blocked with 5% Non‐Fat Milk (#A600669, Sango Biotech) for 1 hour at room temperature and incubated with specific antibody at 4°C overnight followed by HRP‐conjugated secondary antibody incubation for 1 hour at room temperature. Images of membrane with proteins were taken with imager (Bio‐rad ChemiDoc MP, Bio‐rad). The antibodies are recorded: anti‐GAPDH (#60004‐1‐lg, Proteintech), anti‐YTHDF2 (#24744‐1‐AP, Proteintech), anti‐E‐cadherin (#20874‐1‐AP, Proteintech), anti‐N‐cadherin (#66219‐1‐AP, Proteintech), anti‐VIMENTIN (#10366‐1‐AP, Proteintech), anti‐MMP9 (#10375‐2‐AP, Proteintech), anti‐MMP2 (#10373‐2‐AP, Proteintech), anti‐SNAIL (#13099‐1‐AP, Proteintech), anti‐CDK6 (#14052‐1‐AP, Proteintech), anti‐CCND1 (#26939‐1‐AP, Proteintech), anti‐CDK4 (#11026‐1‐AP, Proteintech), anti‐KLF4 (#12173S, Cell Signaling Technology), anti‐SLUG (#C1967, Cell Signaling Technology), anti‐METTL3 (#ab195352, Abcam) and anti‐SETD7 (#ab14820, Abcam).
Xie H., Li J., Ying Y., Yan H., Jin K., Ma X., He L., Xu X., Liu B., Wang X., Zheng X, & Xie L. (2020). METTL3/YTHDF2 m6A axis promotes tumorigenesis by degrading SETD7 and KLF4 mRNAs in bladder cancer. Journal of Cellular and Molecular Medicine, 24(7), 4092-4104.
+ Open protocol
+ Expand
5

Immunohistochemistry Analysis of Xenograft Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
The formalin-fixed paraffin-embedded xenograft tumor tissues were immunohistochemically stained. Next antigen retrieval and non-specific antigen blocking, section were incubated with the first antibody for overnight at 4 °C. Added the second antibody and incubated 30–40 min. DAB plus kit (MaiXin, Fuzhou,China) was used to develop the staining. The nuclear amount of proteins was analyzed with Aperio Nuclear v.9 software. Statistical analyses were performed with GraphPad Prism 7.0. Proteins were detected by using anti-Ki-67 (Proteintech), anti-FAM84B (Proteintech, 18421-1-AP), anti-NPM1 (Proteintech, 60096-1-Ig), anti-CCND1 (Proteintech, 60186-1-Ig), anti-CDK4 (Proteintech, 11026-1-AP).
Wang F., Cheng C., Wang X., Chen F., Li H., Zhou Y., Wang Y., Hu X., Kong P., Zhang L., Cheng X, & Cui Y. (2022). Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma. Cell Death Discovery, 8, 182.
+ Open protocol
+ Expand
6

Immunohistochemical Analysis of Tumor Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumour graft samples anatomized from mice were analysed by immunohistochemical staining. The associated antibodies are as follows: anti‐Ki‐67, anti‐CCND1, anti‐NOP14 (Proteintech) and anti‐DNMT3B (Cell Signaling Technology). Antigen retrieval was performed by heating the slides in sodium citrate buffer (10 mmol/L, pH 6.0). After blocking with bovine serum albumin (Sango Biotech, Shanghai, China), the slides were incubated with anti‐Ki‐67, anti‐CCND1, anti‐NOP14 (Proteintech) and anti‐DNMT3B (Cell Signaling Technology) overnight at 4°C. The slides were then incubated with the secondary antibody goat anti‐rabbit HRP (Cell Signaling Technology) conjugate for 1 hours at room temperature. A DAB solution was used for brown colour development. The strength of positivity was used to semiquantify the strength of positivity, which considered the intensity of the staining and the percentage of positive cells per the formula.
Ying Y., Li J., Xie H., Yan H., Jin K., He L., Ma X., Wu J., Xu X., Fang J., Wang X., Zheng X., Liu B, & Xie L. (2020). CCND1, NOP14 and DNMT3B are involved in miR‐502‐5p–mediated inhibition of cell migration and proliferation in bladder cancer. Cell Proliferation, 53(2), e12751.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!