Chicken (Gallus gallus domesticus) eggs (Winter Egg Farm, Cambridge, UK) were incubated in a digital cabinet incubator (OVA Easy 380, Brinsea) at 38 degrees. At Hamburger Hamilton developmental stage 19 (HH19), the eggshell was fenestrated, the window covered with parafilm (VWR) and eggs were placed horizontally in the incubator. At HH35, epicardial cells were transplanted in PSC with matrigel (1:2 dilution) onto the chorionic chicken vasculature. HESCs were fully differentiated to epicardial cells before administration into the chicken embryos. The eggs were then returned in the incubator until stage HH40. The matrigel plugs were harvested and fixed in 4% paraformaldehyde before being stained with anti-HLA1 (abcam) anti-SM22alpha (Abcam) and sambucus nigra lectin (Vector laboratories).
Anti sm22alpha
Manufactured by Abcam
Anti-SM22alpha is a primary antibody that targets the SM22alpha protein. SM22alpha is a cytoskeleton-associated protein involved in the regulation of smooth muscle cell function. The antibody can be used for the detection of SM22alpha in various applications, such as Western blotting and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti sm22alpha
1
Epicardial Cell Transplantation in Chicken Embryos
2
Epicardial Cell Transplantation in Chicken Embryos
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Chicken (Gallus gallus domesticus) eggs (Winter Egg Farm, Cambridge, UK) were incubated in a digital cabinet incubator (OVA Easy 380, Brinsea) at 38 degrees. At Hamburger Hamilton developmental stage 19 (HH19), the eggshell was fenestrated, the window covered with parafilm (VWR) and eggs were placed horizontally in the incubator. At HH35, epicardial cells were transplanted in PSC with matrigel (1:2 dilution) onto the chorionic chicken vasculature. HESCs were fully differentiated to epicardial cells before administration into the chicken embryos. The eggs were then returned in the incubator until stage HH40. The matrigel plugs were harvested and fixed in 4% paraformaldehyde before being stained with anti-HLA1 (abcam) anti-SM22alpha (Abcam) and sambucus nigra lectin (Vector laboratories).
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