Fei 268d morgagni transmission electron microscope

Ultrathin Sects. (90 nm thick) for transmission electron microscopy were taken with a Leica UCT ultramicrotome (Leica Microsystems) and stained with a saturated methanolic solution of 0.5% (w/v) uranyl acetate (Sigma-Aldrich) followed by incubation in 0.5% (w/v) lead citrate (Sigma-Aldrich) (Reynolds 1963 (link)). They were examined using an FEI 268D ‘Morgagni’ transmission electron microscope (FEI Company, Hillsboro, OR, USA) equipped with an Olympus-SIS ‘Morada’ digital camera (Olympus). The samples were collected in five independent randomly selected repeats.

Ultrathin sections (90 nm thick) were taken with a Leica UCT ultramicrotome (Leica Microsystems) and stained with saturated solution of methanolic solution of 0.5% (w/v) uranyl acetate (Sigma–Aldrich) followed by incubation in 0.5% (w/v) lead citrate (Sigma–Aldrich). Examinations were made on an FEI 268D “Morgagni” transmission electron microscope (FEI Company, Hillsboro, OR, USA) equipped with an Olympus-SIS “Morada” digital camera (Olympus). Samples were collected in five independent randomly selected repeats.

The analyses of hepatocyte ultrastructure were performed on liver fragments which were fixed in 2% (w/v) of paraformadehyde and 2.5% (v/v) of glutaraldehyde solution in a 0.05 M cacodylate buffer (pH 7.2) for 2 h. The samples were rinsed 4 times with cacodylate buffer (pH 7.2) at the same concentration. They were then post-fixed for 1 h at 4 °C in a 2% (w/v) solution of osmium tetroxide dissolved in a 0.05 M cacodylate buffer. The samples were dehydrated in increasing concentrations of ethanol (from 10 to 100%) and finally substituted with pure propylene oxide. They were infiltrated with an epoxy resin of medium hardness (resin composition for 100 mL: 45.6 mL of glycid ether, 30.8 mL of DDSA, 23.5 mL of MNA, and 1.5 mL of DMP-30). The resin was polymerized for 24 h at 60 °C in silicone flat embedding molds. The ultra-thin (80 nm thick) sections for transmission electron microscopy analysis were made using the Leica UCT ultramicrotome (Leica Microsystems, Wetzlar, Germany). The material was contrasted in 1.2% (w/v) of uranyl acetate solution (Sigma-Aldrich, St. Louis, MO, USA) for 10 min and saturated lead citrate solution (Sigma-Aldrich, St. Louis, MO, USA) for 10 min. Sections were examined on an FEI 268D “Morgagni” transmission electron microscope (FEI Company, Hillsboro, OR, USA) equipped with an Olympus-SIS “Morada” digital camera (Olympus, Münster, Germany).