Ow cytometry

Manufactured by BD

Sourced in United States, China

Flow cytometry is an analytical technique used to measure and analyze the physical and chemical characteristics of cells or particles in a fluid sample as they pass through a laser beam. It can be used to count, sort, and characterize different cell types within a mixed population.

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Lab products found in correlation

Annexin 5 fitc, by BD (5 mentions) Cellquest software, by BD (4 mentions) Fitc annexin 5 apoptosis detection kit, by BD (4 mentions) Cell cycle detection kit, by Keygen Biotech (4 mentions) Pbs buffer, by Thermo Fisher Scientific (4 mentions) Annexin 5 fitc pi apoptosis detection kit, by BestBio (2 mentions) Ros assay kit, by Beyotime (2 mentions) Binding buffer, by Thermo Fisher Scientific (2 mentions) Propidium iodide, by Thermo Fisher Scientific (2 mentions) Annexin 5, by Thermo Fisher Scientific (2 mentions) Propidium iodide, by BD (2 mentions) Annexin 5 fitc apoptosis detection kit, by Keygen Biotech (2 mentions) Dnase 1, by AppliChem (2 mentions) Heparin anti coagulated vacutainer tubes, by BD (2 mentions) Collagenase type 4, by Merck Group (2 mentions) Dcfh da, by Beyotime (1 mentions) Ow cytometer, by BD (1 mentions) Cell cycle analysis kit, by Beyotime (1 mentions) 6 well plate, by Corning (1 mentions) Propidium iodide, by Merck Group (1 mentions)

Spelling variants of this product

FACSCalibur ow cytometry (9 citations) FACScan® ow cytometry (5 citations) Ow cytometry analysis (4 citations) BD FACS Calibur ow cytometry system (3 citations) FACS-Canto II ow cytometry (3 citations) Accur C6 Plus ow cytometry (2 citations) FACSAria ow cytometry (2 citations) Ow cytometry antibodies (2 citations) Ow cytometry FACSCalibur instrument (2 citations)

89 protocols using ow cytometry

Characterizing Microglial Activation and Neuroprotection

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The supernatants of BV2 cells were harvested. The blood of mice was collected from the eyeballs and centrifuged to collect serum. According to the manufacturer's protocol, the NGF, IL-1β, and IL-10 protein concentrations in BV2 cells and serum were determined by ELISA. Brie y, samples were assayed at 450 nm, and the concentrations were calculated from respective standard curves.
Flow Cytometric Analysis BV2 cells were harvested by centrifuging after washing the BV2 cells with cold PBS. Next, BV2 cells were stained with anti-mouse CD86-FITC or anti-mouse CD206-APC followed standard protocols and manufacturer's instructions. Finally, the stained cells were immediately analyzed by ow cytometry (BD, USA). PC-12 cells were harvested and washed with cold PBS, followed by the addition of 195 μL binding buffers. Then incubate the cells with 5 μL Annexin V-FITC for 10 min and 10 μL PI for 5 min at room temperature in the dark. Finally, the stained cells were immediately analyzed by ow cytometry (BD, USA).
CCK-8 Assay PC-12 cells with or without Aβ1-42 were cultured with the supernatant of different groups of BV2 cells in 96-well plates. Then, 10 μL CCK-8 reagents per well were added to the cells at 37°C for 2 h, and the absorbance at 450 nm was measured to calculate the cell viability using a multi-mode reader (LD942, Beijing, China).

Jiao L., Liu M., Zhong X., Yao W., Ma G., Shen J., Wu Y., Du K., Liu J., Tong J., Liu C., Fu J., Yu Z., & Wei M. (2021). Cordycepin Improved Neuronal Synaptic Plasticity Through CREB-Induced NGF Up-Regulation Driven By Microglial M2 Polarization: A Symphony Communication Between Microglia and Neurons in AD.

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Annexin V-FITC Apoptosis Assay

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Following treatment, cell apoptosis was assessed using an Annexin V-FITC apoptosis detection kit (BD Pharmingen, San Diego, CA, USA). In brief, Annexin V‐FITC (5 μl) and PI (5 μl) were added to 100 μl cells at a concentration of 1x106 cells/ml and incubated in the dark for 15 min at room temperature. Subsequently, binding buffer was added, and apoptosis was analyzed using ow cytometry (BD Biosciences).

Cao Y., Dai F., Li Y., Jia L., Luan Y, & Zhao Y. (2018). The research on the mechanism of Tsoong inhibiting for colon cancer. Saudi Journal of Biological Sciences, 26(3), 605-613.

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Measuring Intracellular ROS in CRC Cells

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The levels of ROS were measured using DCFH-DA (Beyotime, Shanghai, China) according to the manufacturer's protocol. Brie y, CRC cells were treated with α-hederin or not. Next, cells were labeled with 20 µM DCFH-DA and incubated at 37°C for 30 min in the dark. Following the incubation, the uorescence intensity of DCF was measured using ow cytometry (BD Biosciences, US).

Wang G., Zhu Z.M, & Wang K. (2024). Identification of ROS and KEAP1-related genes and verified targets of α-hederin induce cell death for CRC. Drug development research, 85(3).

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Cell Cycle and Apoptosis Assays

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For the cell cycle assay, after LIPUS or sham irradiation treatment, cell-laden GelMA scaffolds were cut into small pieces and lysed using GelMA Lysis Buffer (EFL-GM-LS-001, SuZhou, China), harvested, and xed with pre-cooled 75% ethanol at 4 °C overnight. After centrifugation, the cells were incubated with PI and RNase A at 37 °C for 30 min in the dark. The cell cycle stages were analyzed at an excitation wavelength of 488 nm using ow cytometry (BD Biosciences, San Jose, USA) and the data were analyzed using FlowJo software (TreeStar, Ashland, OR, USA).
Apoptosis of H9C2 cells was determined using an FITC Annexin V and PI double staining kit (Servicebio, Wuhan, China). After different treatments, the cells were collected, resuspended, and incubated with 5 µL of Annexin V and 5 µL of PI. After incubation for 20 min in the dark at room temperature, the cells were stained for 15 min, and a ow cytometer (BD Biosciences, San Jose, USA) was used to detect apoptosis. The data were analyzed using FlowJo software (TreeStar, Ashland, OR, USA).

Hu Y., Jia Y., Wang H., Cao Q., Yang Y., Zhou Y., Tan T., Huang X, & Zhou Q. (2022). Low-intensity pulsed ultrasound promotes cell viability and inhibits apoptosis of H9C2 cardiomyocytes in 3D bioprinting scaffolds via PI3K-Akt and ERK1/2 pathways. Journal of biomaterials applications, 37(3).

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Cell Cycle Analysis by Flow Cytometry

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Cell Cycle Analysis Kit (Beyotime) was used for analysis of cell cycle distribution. Transfected cells were collected and xed with ice-cold 70% ethanol at 4 ℃ overnight. The next day, cells were incubated with RNase A and PI for 30 minutes. DNA content was measured by ow cytometry (BD Biosciences) and analyzed by FlowJo software.

Liu S., Wu X., Li F., Yao W., Wu Z., Dong P., Wang X., & Gong W. (2020). Estrogen-Related Receptor-α Promotes Pancreatic Cancer Progression by Enhancing the Transcription of PAI1 and Activating the MEK/ERK Signaling Pathway.

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Mitochondrial Membrane Potential Modulation

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Mitochondrial membrane potential (MMP) of cells was detected by JC-1. Cells in the 6-well plate were incubated with 10 4.4 TCID 50 PCV2 for 2 h and discarded the supernatant, then Cepharaanthine and Curcumin were added. After 48 h incubation, cells were stained with JC-1 using a commercial kit (Beyotime Biotechnology, Jangsu, China) treated and analyzed by ow cytometry (BD Biosciences, USA).

Xu Y., Zheng J., Sun P., Guo J., Zheng X., Sun Y., Fan K., Yin W.H., Li H., & Sun N. (2020). Cepharanthine and Curcumin inhibited mitochondrial apoptosis induced by PCV2.

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Cell Cycle and Apoptosis Analysis in SiHa Cells

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For cell cycle analysis, SiHa cells were incubated with various concentrations of 4SC-202 (0, 1, and 5 μM) for 72 h, digested with 0.25% trypsin and resuspended in phosphate-buffered saline (PBS) at a density of 1 × 10 6 cells/mL. Cell cycle distribution was detected by ow cytometry (BD Biosciences, CA, USA) and analyzed using Cell Quest software. For apoptosis detection, the cell suspension was gently mixed with 5 μL Annexin V-FITC and 10 μL PI, followed by an incubation at room temperature for 15 min in the dark. Apoptosis rate was determined by using the ow cytometry.

Zhang H., Li M., Sun H., Yang W., Ye M., Li H, & Meng Y. (2022). 4SC-202 exerts an anti-tumor effect in cervical cancer by targeting PRLR signaling pathway. Journal of molecular histology, 53(6).

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Cell Cycle and Apoptosis Analysis of Glioma Cells

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In the cell cycle analysis, U87MG and A172 glioma cells were collected, re-suspended and stained with propidium iodide (PI) (BD Biosciences; USA) for 20 min in the presence of RNase A. In the apoptosis assay, U87MG and A172 cells were stained with Annexin V-FITC (BD Biosciences; USA) and PI for 20 min. Cells were analyzed using ow cytometry (BD Biosciences; USA) according to manufacturer's instructions.

Sun Z., Xue H., Yan W., Wang S., Xu J., Qian M., Wang C., Zhao R., Wang C., & Li G. (2020). Ribosomal Protein S27-Like is overexpressed in glioma cells and inhibition of RPS27L expression suspends the tumorigenesis.

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Transfection Efficiency Comparison in 293T and SMMC-7721 Cells

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Human embryonic kidney cell 293T cells, liver cancer cell line SMMC-7721 were preserved by the laboratory. And cultured in Dulbecco's Modi ed Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL - 1 penicillin and 100 µg/mL streptomycin in a humidi ed incubator with 5% CO 2 at 37 °C. SMMC-7721 cells were seeded at 2 × 10 5 cells/well in 6-well plate (Corning Inc., NY, NJ, USA) in 2 mL of complete medium. After 24 hours of incubation, the medium in each well was replaced with 2 mL of fresh serum-free medium. The pVAX-GFP (pGFP) reporter plasmid was maintained at 2 µg per well, while the mass ratios of PL/pGFP, Tf-PL/pGFP and Lipo 2000/pGFP were 25:1. The serum-free medium was then replaced with complete medium after 6 hours. Then, the cells were cultured for an additional 48 hours at 37 °C. Expression of GFP was visualized by an Olympus IX 71 inverted uorescence microscope (Olympus Corp., Tokyo, Japan). Cell suspensions were harvested and analyzed by ow cytometry (BD Biosciences, San Jose, CA, USA) to determine transfection e ciency.

Wang K., Shang F., Chen D., Jiao J., Cao T., Wang X., He S., & Liang X. (2020). Targeted Protein Liposomes-Mediated AChE Gene Therapy for Effective Liver Cancer Treatment.

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Cell Cycle Analysis by Flow Cytometry

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The analysis of cell cycle was performed by a ow cytometry (BD, San Diego, CA). Cells were treated by DPP supplemented with or without CM for 24h, and then the cells were harvested, xed in cold ethanol, and stored at -20°C overnight. The cell cycle was analyzed by ow cytometry using after the xed cells were suspended in PI/RNase staining buffer.

Qiao L., Li R., Wu Y., Wang Y., Wang B., & Wang G. (2020). Cardamonin Enhances Cisplatin Chemosensitivity of Nasopharyngeal Cancer Cells via Attenuating c-Myc-Mediated β-Catenin/ABCG2 Signaling.

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