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Nod scid

Manufactured by GemPharmatech
Sourced in China

The NOD/SCID is a laboratory mouse model that lacks functional T cells, B cells, and natural killer cells. It is widely used in research to study human diseases and to evaluate the efficacy of new therapies.

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2 protocols using nod scid

1

NOD/SCID Mice for Biomedical Research

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Four- to five-week-old, male non-obese diabetic/severe combined immunodeficient (NOD/SCID) and NOD/SCID/IL-2γ-receptor null (NSG) mice were purchased from Gempharmatech (Nanjing, China). All mice were maintained with 12-h light-dark cycles under specific pathogen-free conditions. All procedures were carried out in accordance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. Experiments were conducted with the approval of the Ethics Committee of the First Affiliated Hospital, Zhejiang University School of Medicine and the Animal Care Committee of Zhejiang University School of Medicine.
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2

Characterization of CRC Cell Lines

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The CRC cell lines MC38, CT26, and HT‐29 were purchased from the American Type Culture Collection (ATCC) and cultured according to the manufacturer's instructions. These cells were characterized using short tandem repeat markers by Genewiz, Inc. and were confirmed to be mycoplasma free (last tested in 2017). Note that 5‐ to 6‐week‐old BALB/c nude, C57BL/6, BALB/c, NOD/SCID, and NOD‐Prkdcem26Cd52IL2rgγem26Cd52/Gpt (NCG) mice were purchased from Gempharmatech and were housed under pathogen‐free conditions, in temperature‐controlled holding rooms (20−22°C), with 30%−34% humidity, and on a 12 h on (6 a.m.) and off light cycle (6 p.m.).
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