Paramecia were collected by centrifugation at 3,000 rmp for 5 min and quantified by optical density measurement at OD500 nm as described (45 (link)). The paramecia were diluted to a density of 1000 paramecia per ml and incubated with 4-(4-(didecylamino)styryl)-N-methylpyridinium iodide (4-Di-10-ASP) at 25 μg/ ml for 1 hr (Invitrogen, Carlsbad, CA, USA). The paramecia were then washed with water three times for 10 min each time to remove the residual 4-Di-10-ASP. Fish larvae were fed with 4-Di-10-ASP labeled or unlabeled (control) paramecia at 1000 paramecia/ml for two hours. The fish larvae were photographed immediately using a GFP2 filter on a MZ16F stereoscopic microscope (Leica Microsystems). The images were analyzed using Image J software to get the fluorescence intensity.
Mz16f stereoscopic microscope
Manufactured by Leica
The Leica MZ16F stereoscopic microscope is a precision instrument designed for detailed observation and analysis. It features a high-quality optical system that provides crisp, high-resolution images. The microscope is equipped with a magnification range to accommodate a variety of sample sizes and applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mz16f stereoscopic microscope
1
Paramecia Labeling and Fish Larvae Feeding
2
Zebrafish Embryo Exposure to Eriocitrin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zebrafish embryos were exposed to eriocitrin from 6 hpf to 5 dpf. The number of survivors was counted under a MZ16F stereoscopic microscope (Leica Microsystems). One hour before evaluation of locomotor activity, a fish was removed from each experimental unit and placed in a well with 50 μl of E3 medium in a 96-well, clear-bottom plate for acclimatization. The fish were then video-recorded for 15 min. The resulting footage was evaluated to measure the swimming distance using the EthoVision XT system ver8.0 (Noldus Information Technology, Wageningen, The Netherlands) as described previously60 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!