Binding of human anti-HBs and control monoclonal IgG antibodies (mGO53 and ED38; Meffre et al., 2004 (link); Wardemann et al., 2003 (link)) to HEp-2 cell–expressing autoantigens was analyzed at 50 µg/ml by ELISA (AESKULISA ANA-HEp-2, Aesku.Diagnostics), and indirect immunofluorescence assay (IFA; ANA HEp-2 AeskuSlides, Aesku.Diagnostics) following the manufacturer’s instructions. IFA sections were examined using the fluorescence microscope Axio Imager 2 (Zeiss), and pictures were taken at magnification ×40 with 5,000 ms-acquisition using ZEN imaging software (Zen 2.0 blue version, Zeiss) at the Photonic BioImaging platform (Institut Pasteur).
Zen imaging software zen 2.0 blue version
Manufactured by Zeiss
ZEN imaging software is a comprehensive platform for microscopy image acquisition, processing, and analysis. It provides a user-friendly interface for managing and manipulating microscopy data, enabling researchers to efficiently work with their samples and generate high-quality images.
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Lab products found in correlation
4 protocols using zen imaging software zen 2.0 blue version
1
Autoantigen Binding Assay for Anti-HBs
2
Immunofluorescence Assay of SARS-CoV-2 Antibodies
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Recombinant SARS-CoV-2 S–specific and control IgG antibodies (mGO53 and ED38) at 100 µg/ml were analyzed by indirect immunofluorescence assay on HEp-2 cells sections (ANA HEp-2 AeskuSlides; Aesku.Diagnostics) using the kit’s controls and FITC-conjugated anti-human IgG antibodies as the tracer according to the manufacturer’s instructions. HEp-2 sections were examined using the fluorescence microscope Axio Imager 2 (Zeiss), and pictures were taken at a magnification ×40 with 5,000 ms-acquisition using ZEN imaging software (Zen 2.0 blue version; Zeiss) at the Imagopole platform (Institut Pasteur).
3
IgG Antibody Binding to Bacillus subtilis
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Recombinant IgG antibodies (150 µg/ml final concentration) were assayed by indirect immunofluorescence assay (IFA) using the ANA HEp-2 AeskuSlides® kit (Aesku.Diagnostics) following the manufacturer’s instructions. mGO53, ED38 and kit’s control antibodies were included in each experiment. Binding of human IgG antibody L2-168 to B. subtilis was tested by IFA. Briefly, fixed bacteria were 1:10-diluted in PBS and incubated overnight on Poly-L-Lysine-treated Superfrost Plus Slides (Fischer Thermo Scientific). Slides were then washed twice with PBS and incubated with L2-168 at 10 µg/ml for 1 h at room temperature. After washing steps, FITC-conjugated anti-human IgG antibodies from ANA HEp-2 AeskuSlides® kit was used as tracer for revealing bacteria binding. IFA slides were examined using the fluorescence microscope Axio Imager 2 (Zeiss), and pictures were taken at magnification ×100 with 5000 ms-acquisition using ZEN imaging software (Zen 2.0 blue version, Zeiss) at the Imagopole platform (Institut Pasteur).
4
Binding of Anti-HIV-1 Env Antibodies to HEp-2 Cells
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Binding of human anti-HIV-1 Env and control monoclonal IgG antibodies (mGO53 [Wardemann et al., 2003 (link)] and ED38 [Meffre et al., 2004 (link)]) to HEp-2 cell–expressing autoantigens was analyzed at 100 µg/ml by indirect IFA (ANA HEp-2 AeskuSlides; Aesku.Diagnostics) following the manufacturer’s instructions in two independent experiments. IFA sections were examined using the fluorescence microscope Axio Imager 2 (Zeiss), and pictures were taken at magnification 40× with 5,000 ms–acquisition using ZEN imaging software (Zen 2.0 blue version; Zeiss) at the Photonic BioImaging platform (Institut Pasteur).
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