Ethanol (95% v/v, USP) was diluted to 20% (v/v) with rodent drinking water from the Laboratory Animal Resources Center, Texas Tech University Health Sciences Center, Lubbock TX. Tetracycline derivatives and their properties are listed in Table 1 . All tetracyclines used in our study have been approved by the FDA as antibiotics in doses varying up to 500 mg, although some are no longer used (see: http://www.accessdata.fda.gov/scripts/cder/drugsatfda/ ). Drugs were obtained from commercial sources and stored according to the vendor's recommendations. Minocycline HCl (#M9511), chlortetracycline HCl (#46133-Fluka), oxytetracycline HCl (# D9811), tigecycline hydrate (#PZ0021), and tetracycline HCl (#T3383) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Doxycycline HCl (#SC337691) and demeclocycline HCl (#SC204710A) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA) and tigecycline (base) (#15026) was obtained from Cayman Chemical (Ann Arbor, MI, USA). Doses of 0, 20, 40, 60, 80, 100 and 120 mg/kg were made fresh and used within 25 hours.
Oxytetracycline hcl
Manufactured by Merck Group
Sourced in United States
Oxytetracycline HCl is a chemical compound used in laboratory settings. It is a crystalline powder that serves as a versatile reagent for various analytical and experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Xylenol, by Merck Group (2 mentions)
Tigecycline hydrate, by Merck Group (1 mentions)
Minocycline hcl, by Merck Group (1 mentions)
Sp8 mp, by Leica camera (1 mentions)
Alizarin red s, by Merck Group (1 mentions)
2 acrylamido 2 methylpropane sulfonic acid amps, by Merck Group (1 mentions)
Milli q, by Merck Group (1 mentions)
Ethylene glycol dimethacrylate egdma, by Merck Group (1 mentions)
Sodium chloride nacl, by ITW Reagents (1 mentions)
4 protocols using oxytetracycline hcl
1
Tetracycline Antibiotic Formulations and Dosing
2
Fluorescent Bone Marker Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
During the 12 weeks healing period, fluorescent bone markers were injected 55 (link) to observe the dynamics of new bone formation. Three weeks after the operation, 20 mg alizarin red S/kg of body weight was administered intraperitoneally (IP) (Sigma-Aldrich), 20 mg oxytetracycline HCl/kg of body weight (Sigma-Aldrich) was injected IP at 6 weeks post-surgery, and 20 mg xylenol/kg of body weight (Sigma-Aldrich) was injected IP at 9 weeks post-surgery. All dyes were prepared immediately before use in saline.
3
Visualizing Bone Remodeling Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 8 weeks of healing, the dynamics of the newly formed bones were observed by injecting them with fluorescent bone marker labels. At 2 weeks, alizarin red S (20 mg/kg body weight), oxytetracycline HCl (20 mg/kg body weight), and xylenol (20 mg/kg body weight) (Sigma-Aldrich) were administered via intraperitoneal injection. All dyes were prepared with saline immediately before use. To visualize remodeled bone, an intravital multiphoton microscope (SP8-MP; Leica, Wetzlar, Germany) with a water immersion lens (25×, 0.9 NA) was used. The fluorophores were excited using the InSight DS Plus laser system (Spectra-Physics, Santa Clara, USA) at the following wavelengths: xylenol (excitation = 800 nm, emission = 375-570 nm), oxytetracycline (excitation = 1040 nm, emission = 365-490 nm), and alizarin (excitation = 1180 nm, emission = 538-580 nm). Alizarin -, oxytetracycline-, and xylenol-positive areas were quantified using the ImageJ software. All values are expressed as mean ± SD (n = 4).
4
Synthesis of Polymeric Adsorbent Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oxytetracycline HCl ( 95%), 2-acrylamido-2-methylpropane sulfonic acid (AMPS) and ethylene glycol dimethacrylate (EGDMA) were from Sigma-Aldrich (St. Louis, Mo, USA); 1,1´-azobis(isobutyronitrile) (AIBN) from Acros (Geel, Belgium); 2hydroxyethyl methacrylate (HEMA) and hydrochloric acid from Merck (Darmstadt, Germany); dimethylsulfoxide (DMSO) from Scharlau Chemicals (Barcelona, Spain); methanol HPLC gradient from Prolabo (Barcelona, Spain); and sodium chloride (NaCl) from Panreac (Barcelona, Spain). Purified water was obtained by reverse osmosis (MilliQ®, Millipore).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!