Glutathione S-transferase (GST)-pro-IL1α and GST-pro-IL1β fusion proteins purified from a wheat germ expression system were purchased from Abnova (Taipei, Taiwan), recombinant human IL-1α and IL-1β from Peprotech (Rocky Hill, New Jersey), thapsigargin from Abcam (Cambridge, MA), and N-formyl-methionyl-leucyl-phenylalanine (fMLP) from Sigma-Aldrich (St. Louis, MO). Anti-IL-1α-neutralizing antibody (MABp1) and IgG isotype control were obtained from Dr. John Simard at XBiotech (Austin, TX) and anti-IL-1β-neutralizing antibody (canakinumab) from Dr. Hermann Gram at Novartis (Basel, Switzerland). Anti-TF-neutralizing antibody (TF8-5G9) was provided by Drs. James Morrisey and Kevin Croce. CatG from human neutrophils was purchased from Sigma-Aldrich, CatG inhibitor I from Cayman Chemical Co. (Ann Arbor, MI), CatG substrate from Bachem (Torrance, CA), and NE substrate from EMD Millipore (Billerica, MA).
Recombinant human il 1α and il 1β
Manufactured by Thermo Fisher Scientific
Sourced in United States
Recombinant human IL-1α and IL-1β are cytokines produced through recombinant DNA technology. They are involved in the regulation of immune and inflammatory responses.
Automatically generated - may contain errors
Lab products found in correlation
N formyl methionyl leucyl phenylalanine, by Merck Group (1 mentions)
Thapsigargin, by Abcam (1 mentions)
P38 inhibitor sb202190, by Bio-Techne (1 mentions)
Nf κb inhibitor bay 11 7082, by Santa Cruz Biotechnology (1 mentions)
Jnk inhibitor sp600125, by Bio-Techne (1 mentions)
Tlr4 ligand lipopolysaccharide lps, by Merck Group (1 mentions)
U0126, by Cell Signaling Technology (1 mentions)
2 protocols using recombinant human il 1α and il 1β
1
Purification and Neutralization of Interleukin-1
2
Signaling Pathway Modulation in Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Rabbit anti-human A20 and GAPDH antibodies, rabbit anti-human phosphorylated ERK, JNK, p38, and NF-κB P65 antibodies, and the ERK inhibitor U0126, were purchased from Cell Signaling Technology (Beverly, MA, USA). The NOD2 ligand MDP was purchased from Invivogen (San Diego, CA, USA). The TLR4 ligand lipopolysaccharide (LPS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Human IL-8 and IL-1β ELISA kits were purchased from Jiamay Biotech. (Beijing, China). The A20 expression plasmid was purchased from GeneCopoeia (Germantown, MD, USA). The JNK inhibitor SP600125 and P38 inhibitor SB202190 were purchased from Tocris (Bristol, UK). The mouse anti-human NOD2 antibody and NF-κB inhibitor Bay 11-7082 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Recombinant human IL-1α and IL-1β were purchased from PeproTech Inc. (Rocky Hill, NJ, USA). NF-κB inhibitor BAY 11-7085 was purchased from MedChemExpress (Monmouth Junction, NJ, USA).
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