AzA was purchased from TCI Chemicals Pvt. Ltd., Chennai, India. TTO (Melaleuca alternifolia) was purchased from Allin Exporters, Noida, India. Phospholipipon 90G (PL-90G) was supplied ex gratis by Phospholipid GmbH, Germany. Dimethyl sulfoxide (DMSO), isopropyl myristate (IPM), Tween 80, Carbopol 934, PEG 400, triethanolamine (TEA), acetonitrile (ACN), sodium dihydrogen orthophosphate, and orthophosphoric acid were purchased from Loba Chemie Pvt. Ltd., Mumbai, India. Absolute ethanol was purchased from Changshu Hongsheng Fine Chemical Co. Ltd., Changshu, China. Nutrient broth, Agar, Brain heart infusion (BHI), Erythromycin, and Resazurin Dye were purchased from Himedia Labs Pvt. Ltd., Kolkata, India. In the study, the commercial formulation of azelaic acid was Aziderm 10% w/w gel (Micro labs Ltd. Bangalore, India) procured from a local drug house. Ultrapure water prepared by Micropore™ assembly installed at Shoolini University, Solan, was used throughout the study.
The present investigation was conducted as per the guidelines of CPCSEA (Committee for controlling and supervising experiments on animals). The Institutional Animal Ethical Committee duly approved the experimental protocol of Shoolini University (IAEC/SU/09/18). Male Swiss albino mice and Wistar rats used in the current study were obtained from the central animal house of Shoolini University Solan (HP).
The present investigation was conducted as per the guidelines of CPCSEA (Committee for controlling and supervising experiments on animals). The Institutional Animal Ethical Committee duly approved the experimental protocol of Shoolini University (IAEC/SU/09/18). Male Swiss albino mice and Wistar rats used in the current study were obtained from the central animal house of Shoolini University Solan (HP).