To investigate the impact of GIs on the serum metabolites of chickens, the untargeted serum metabolome profile was determined using liquid chromatography-mass spectrometry (LC-MS) at the Campus Chemical Instrumentation Center, Mass Spectrometry and Proteomics Facility, The Ohio State University (CCIC, MS&P Facility, OSU) (https://live-ccic.pantheonsite.io/MSP ). Metabolome profiling was performed on a Thermo Orbitrap LTQ XL instrument in positive-mode analysis with high-performance liquid chromatography (HPLC) separation on a Thermo Scientific RLCS ultimate 3000 LC system using a Poroshell 120 SB-C18 (2 by 100 mm, 2.7-μm particle size) column. The significantly (P < 0.05, Kruskal-Wallis test) altered metabolites and the associated metabolic pathways were identified using XCMS Online (https://xcmsonline.scripps.edu/ ) and expressed as the fold changes in abundance compared to the abundance in NC (noninfected and nontreated) chickens. Principal-component analysis (PCA) was performed to compare the metabolome profiles between the treatment groups. Progenesis QI software (Waters/Nonlinear Dynamics) was used to validate the results. Details of methods are included in the supplemental material.
Rlcs ultimate 3000 lc system
Manufactured by Thermo Fisher Scientific
The RLCS ultimate 3000 LC system is a high-performance liquid chromatography (HPLC) instrument designed for analytical applications. It provides precise and reliable separation and detection of analytes in complex samples. The system features advanced components and software to ensure consistent and reproducible results.
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2 protocols using rlcs ultimate 3000 lc system
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Serum Metabolome Profiling of Chickens
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Serum Metabolome Profiling of Chickens
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To investigate the impact of GIs on the serum metabolites of chickens, the untargeted serum metabolome profile was determined using liquid chromatography-mass spectrometry (LC-MS) at the Campus Chemical Instrumentation Center, Mass Spectrometry and Proteomics Facility, The Ohio State University (CCIC, MS&P Facility, OSU) (https://live-ccic.pantheonsite.io/MSP ). Metabolome profiling was performed on a Thermo Orbitrap LTQ XL instrument in positive-mode analysis with high-performance liquid chromatography (HPLC) separation on a Thermo Scientific RLCS ultimate 3000 LC system using a Poroshell 120 SB-C18 (2 by 100 mm, 2.7-μm particle size) column. The significantly (P < 0.05, Kruskal-Wallis test) altered metabolites and the associated metabolic pathways were identified using XCMS Online (https://xcmsonline.scripps.edu/ ) and expressed as the fold changes in abundance compared to the abundance in NC (noninfected and nontreated) chickens. Principal-component analysis (PCA) was performed to compare the metabolome profiles between the treatment groups. Progenesis QI software (Waters/Nonlinear Dynamics) was used to validate the results. Details of methods are included in the supplemental material.
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