GH was estimated by electrochemiluminescence immunoassay (ECLIA) (COBAS 600, Roche diagnostics, Germany). IGF1 was measured by ECLIA (Dia-Sorin, Liaison, Germany). HbA1c was estimated by HPLC using ion-exchange chromatography (Bio-Rad Laboratories, USA) with an intra-and inter-assay coefficient of variation 0.58% and 0.49%. Other hormones like serum T4, TSH, LH, FSH, PRL, testosterone/estradiol, and cortisol were assessed by ECLIA. All the samples were thawed only once for biochemical assessment of IL-6; PDGF, and TGF-β1 by utilizing the Sandwich ELISA technique.
Cobas 600
Manufactured by Roche
Sourced in Germany
The COBAS 600 is an automated, high-throughput laboratory analyzer designed for clinical chemistry and immunochemistry testing. It is capable of handling a wide range of sample types and delivering accurate and reliable results. The COBAS 600 is a core component of Roche's diagnostic solutions, focused on providing efficient and effective laboratory testing.
Automatically generated - may contain errors
8 protocols using cobas 600
1
Comprehensive Biochemical Profiling Protocol
2
Blood Profiles in Outdoor Activities
3
Comprehensive Endocrine Biomarker Analysis
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HbA1c was measured by HPLC using ion-exchange chromatography (Bio-Rad Laboratories, USA, intra-and inter-assay CV 0.58% and 0.49%, respectively). All anterior pituitary hormones, plasma C-peptide and insulin were measured by the electrochemiluminescence immunoassay (ECLIA) (COBAS 600, Roche diagnostics, Germany). IGF-1 was measured by ECLIA (Dia-Sorin, Liaison, Germany). GIP (Total), GLP- 1 (Total) and glucagon were measured by sandwich ELISA method using kits manufactured by Millipore Corporation, Billerica, USA. The assay range for GIP, GLP-1 and glucagon assay were 4.2 to 2000 pg/ml, 4.1 to 1000 pM and 0.02 to 2 ng/ml respectively. The intra- and inter assay % CV for GIP, GLP-1 and glucagon assay was 6.7% & 6.1%, 2% & 12%, and 3.09% & 3.06%, respectively.
4
Cardiometabolic Biomarker Assessment Protocol
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Venous blood samples were taken on fasting (abrosia over 12 hours) for fasting blood glucose (FBG), total cholesterol (TCHOL), triglycerides (TGs), LDL, high-density lipoprotein (HDL), blood urea nitrogen (BUN), glycosylated haemoglobin A1C (HbA1c) and Lp-PLA2. Morning urine and 24 h urine samples from all participants were collected to determine levels of albumin, creatinine and the UACR and estimated glomerular filtration rate (eGFR). The 24-h urine protein levels were measured via the trichloroacetic acid method using a photometer [6 (link)]. The serum creatinine concentrations were determined by the Jaffe reaction [7 (link)]. The modification of diet in renal disease (MDRD) formula was used to determine the eGFR [8 (link)]. FBG, TCHOL, TG, LDL, HDL, Cr and BUN were detected by the Roche fully automatic biochemical analyser (Cobas600, Roche, Basel, Switzerland). HbA1c was tested by a high-pressure liquid chromatograph (LC-2030C 3D, Shimadzu Corporation, Kyoto, Japan). Lp-PLA2 was determined by using an enzyme-linked immunosorbent kit (EH304RB, Thermo Fisher, Waltham, MA).
5
Comprehensive Lipid and Enzyme Panel
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Lipids (total cholesterol, triglycerides and phospholipids), Alkaline Phosphatase, ALT, AST, and Bilirubin were measured enzymatically on a Roche Cobas‐600. Plasma lipids were measured enzymatically (Wako Chemicals USA, Inc Richmond, VA) on a ChemWell‐2910 analyzer (Awareness Technology, Inc, Palm City, FL).
6
Fasting Blood Thyroid Hormone Analysis
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Blood samples were drawn from each participant after an overnight fasting period for determination of the circulating levels of thyroid hormones. TSH, free T3 (FT3) and free T4 (FT4) were measured by COBAS 600 (Electrochemiluminescence Technology, Roche Diagnostics, Mannheim, Germany).
7
Serum Cortisol and NSE Analysis
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Blood samples of participants were collected between 7.00 a.m. and 8.00 a.m. before food consumption. Serum cortisol and NSE were determined using an electrochemiluminescence immunoassay technique on a Cobas600 (Roche Diagnostics, IN, USA).
8
Circulating Irisin, Thyroid Hormones, and Metabolic Markers
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All patients, at baseline and then at 2 months of follow-up, underwent blood samplings after an overnight fasting period for determination of the circulating levels of thyroid hormones, serum irisin, glucose, insulin and creatinine and lipid profile. TSH, FT3, FT4 and insulin were measured by COBAS 600 (Electrochemiluminescence Technology, Roche Diagnostics, Mannheim, Germany); reference range for TSH was 0.35–3.2 μU/mL; reference range for FT3 was 2.4–4.2 pg/mL; reference range for FT4 was 8.5–16.5 pg/mL. The homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated according to the following formula: fasting serum insulin (mU/L) × fasting plasma glucose (mmol/L)/22.5. Estimated glomerular filtration rate (eGFR) was assessed by Cockcroft–Gault formula. Irisin concentrations were measured with an enzyme-linked immunosorbent assay (ELISA) kit (measurement range: 0.001–5 μg/mL; sensitivity: 0.001 μg/mL; intrassay variability: 4.86% for 0.678 μg/mL and 7.63% for 1.37 μg/mL: interassay variability: 9.6% for 0.532 μg/mL and 8.02% for 1.14 μg/mL) according to the manufacturer’s directions (Biovendor, laboratory medicine, Czech Republic).
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