Anti rcc 1

Manufactured by Santa Cruz Biotechnology

Anti-RCC-1 is a primary antibody developed by Santa Cruz Biotechnology. It is designed to detect the expression of the RCC-1 (Regulator of Chromosome Condensation 1) protein in biological samples.

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Lab products found in correlation

Goat anti mouse hrp, by Jackson ImmunoResearch (1 mentions) Goat anti rabbit hrp, by Jackson ImmunoResearch (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti tubulin, by Abcam (1 mentions) Anti h2ax, by Merck Group (1 mentions) Anti h3k36me3, by Abcam (1 mentions) Anti v5 tag, by Cell Signaling Technology (1 mentions) Anti p21, by Santa Cruz Biotechnology (1 mentions) Anti h3, by Abcam (1 mentions) Anti myc, by Abcam (1 mentions) Anti ha, by Fortrea (1 mentions) Anti gfp, by Merck Group (1 mentions) Anti ran, by Abcam (1 mentions)

Spelling variants of this product

Anti-RCC-1 (E-6, (2 citations)

2 protocols using anti rcc 1

Western Blotting: Antibody Detection

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Western blotting was performed according to standard laboratory protocols. Antibodies used were: anti-H3K36me3 (Abcam, 9050; 1:1000), anti-H3 (Abcam, 1791; 1:5000), anti-HA (Covance, MMS-101P; 1:2000), anti-H2AX (Millipore 05-636; 1:5000) anti-Tubulin (Abcam, 7291; 1:5000), anti-RCC-1 (Santa Cruz, sc-55559; 1:2000), anti-p21 (Santa Cruz, sc-6246; 1:1000), anti-GAPDH (Santa Cruz, sc-365062; 1:1000), anti-V5-tag (Cell Signaling, 13202; 1:2000), anti-Myc (Abcam, 9106; 1:10000). Secondary antibodies used were: goat anti-mouse HRP (Jackson ImmunoResearch, 115-035-03 or Thermo Fisher Scientific, 31430; 1:5000), goat anti-rabbit HRP (Jackson ImmunoResearch, 111-035-003 or Thermo Fisher Scientific 31460; 1:5000). Uncropped scans of all blots are shown in Supplementary Fig. 12.

Skucha A., Ebner J., Schmöllerl J., Roth M., Eder T., César-Razquin A., Stukalov A., Vittori S., Muhar M., Lu B., Aichinger M., Jude J., Müller A.C., Győrffy B., Vakoc C.R., Valent P., Bennett K.L., Zuber J., Superti-Furga G, & Grebien F. (2018). MLL-fusion-driven leukemia requires SETD2 to safeguard genomic integrity. Nature Communications, 9, 1983.

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Western Blot Antibody Protocol

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Anti-RCC1 is a mouse monoclonal antibody recognizing amino acids 122-421 purchased from Santa Cruz Biotechnology (Cat#: sc-55559) with 1:500 dilution for Western blot analyses. Anti-Ran is a rabbit polyclonal antibody obtained from Abcam (Cat#: ab4781). Anti-GFP is a rabbit antibody from Sigma-Aldrich (Cat#: G1544). Anti-α Tubulin antibody was procured from Calbiochem (Cat#: CP06). It is a mouse monoclonal antibody. All these antibodies used for Western blot analyses or immunofluorescent assays had the dilution factors performed according to the manufacturer's suggestions.

Chen F., Palem J., Balish M., Figliozzi R., Ajavon A, & Hsia S.V. (2014). A Novel Thyroid Hormone Mediated Regulation of HSV-1 Gene Expression and Replication is Specific to Neuronal Cells and Associated with Disruption of Chromatin Condensation. SOJ pharmacy & pharmaceutical sciences, 1(1), 06.

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