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Dm akg

Manufactured by Merck Group
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DM-aKG is a laboratory reagent product manufactured by Merck Group. It is a chemical compound used in various scientific applications and research experiments. The core function of DM-aKG is to serve as a substrate or precursor in biochemical and metabolic processes. No further details on the intended use or specific applications of this product can be provided in an unbiased and factual manner.

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Lab products found in correlation

Gsk j4, by Bio-Techne (2 mentions) Tetracycline, by Merck Group (1 mentions) Cb 839, by Cayman Chemical (1 mentions) Octyl d 2 hg, by LGC (1 mentions) Octyl l 2 hg, by LGC (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) 0.22 μm pore filter, by Merck Group (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions)

5 protocols using dm akg

1

Metabolic Modulation in Fumarate Hydratase Deficiency

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Fh1fl/fl cells were cultured either with 200 µM monomethyl-fumarate (MMF, Sigma-Aldrich) for 2 weeks and then with 400 µM MMF for the following 6 weeks, or with 4 mM monomethyl-succinate (MMS, Sigma-Aldrich) for 8 weeks. HK2 cells were cultured with MMF 400 µM for 8 weeks. Fh1-/- cells were treated with the indicated doses of dimethyl aKG (DM-aKG, Sigma-Aldrich). Fh1 fl/fl cells were treated with histone demethylase inhibitor GSKJ4 (Tocris) 1 µM for 8 weeks. MMF, MMS and GSKJ4 were added twice a week after passaging the cells.
Sciacovelli M., Gonçalves E., Isaac Johnson T., Roberto Zecchini V., da Costa A.S., Gaude E., Vercauteren Drubbel A., Julian Theobald S., Abbo S., Tran M., Rajeeve V., Cardaci S., Foster S., Yun H., Cutillas P., Warren A., Gnanapragasam V., Gottlieb E., Franze K., Huntly B., Richard Maher E., Henry Maxwell P., Saez-Rodriguez J, & Frezza C. (2016). Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition. Nature, 537(7621), 544-547.
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2

Modulation of 2-HG Levels in Cell Lines

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Cell lines were exposed to 1mM or 5mM of dimethyl 2-oxoglutarate (DMaKG; Sigma-Aldrich) for 8-24h, to 20-100uM of octyl-D-2-HG or octyl-L-2-HG (Toronto Research Chemicals) for 8-24h, to 1mM dimethyloxalylglycine (DMOG; Frontier Scientific) for 8-24h, and to CB-839 (Cayman Chemical) for 24h. When appropriate, dimethyl sulfoxide (DMSO; Sigma-Aldrich) was used as vehicle control. In P493-6 cells, MYC suppression was achieved by exposing the cells to 100ng/ml of tetracycline (Sigma-Aldrich).
Qiu Z., Lin A.P., Jiang S., Elkashef S.M., Myers J., Srikantan S., Sasi B., Cao J.Z., Godley L.A., Rakheja D., Lyu Y., Zheng S., Madesh M., Shiio Y., Dahia P.L, & Aguiar R.C. (2020). MYC regulation of D2HGDH and L2HGDH influences the epigenome and epitranscriptome. Cell chemical biology, 27(5), 538-550.e7.
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3

NAC and DM-aKG Media Preparation

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10 mM NAC (Sigma-Aldrich) or 2.5 mM DM-aKG (Sigma-Aldrich) was dissolved into the media and sterilized with 0.22-μm-pore filter (Millipore). The prepared media was used within an hour.
Jeong S., Savino A.M., Chirayil R., Barin E., Cheng Y., Park S.M., Schurer A., Mullarky E., Cantley L.C., Kharas M.G, & Keshari K.R. (2020). High fructose drives the serine synthesis pathway in acute myeloid leukemic cells. Cell metabolism, 33(1), 145-159.e6.
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4

Analyzing AKG and DMAKG Synthesis

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AKG and DMAKG were purchased from Sigma (St. Louis MO, USA). Other chemical reagents were of analytical grade.
Wang Z., Hu J., Faber J., Miszuk J, & Sun H. (2022). Locally Delivered Metabolite Derivative Promotes Bone Regeneration in Aged Mice. ACS applied bio materials, 5(7), 3281-3289.
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5

Metabolic Modulation in Fumarate Hydratase Deficiency

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Fh1fl/fl cells were cultured either with 200 µM monomethyl-fumarate (MMF, Sigma-Aldrich) for 2 weeks and then with 400 µM MMF for the following 6 weeks, or with 4 mM monomethyl-succinate (MMS, Sigma-Aldrich) for 8 weeks. HK2 cells were cultured with MMF 400 µM for 8 weeks. Fh1-/- cells were treated with the indicated doses of dimethyl aKG (DM-aKG, Sigma-Aldrich). Fh1 fl/fl cells were treated with histone demethylase inhibitor GSKJ4 (Tocris) 1 µM for 8 weeks. MMF, MMS and GSKJ4 were added twice a week after passaging the cells.
Sciacovelli M., Gonçalves E., Isaac Johnson T., Roberto Zecchini V., da Costa A.S., Gaude E., Vercauteren Drubbel A., Julian Theobald S., Abbo S., Tran M., Rajeeve V., Cardaci S., Foster S., Yun H., Cutillas P., Warren A., Gnanapragasam V., Gottlieb E., Franze K., Huntly B., Richard Maher E., Henry Maxwell P., Saez-Rodriguez J, & Frezza C. (2016). Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition. Nature, 537(7621), 544-547.
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