Blood was collected by cardiac puncture and left at room temperature for 20 min in a BD Microtainer Serum Separator Tube (BD Biosciences). After centrifugation at 3,000 × g for 20 min, serum samples were subjected to measurement of blood urea nitrogen and creatinine using a Catalyst Dx Chemistry Analyzer system (IDEXX Laboratories, Inc., Westbrook, ME, USA). A tumor necrosis factor (TNF)-α kit was purchased from Abcam (ab208348, Mouse TNF alpha ELISA Kit; Abcam), and the concentrations were measured as described in the manufacturer’s instructions.
Catalyst dx chemistry analyzer system
Manufactured by IDEXX
Sourced in United States
The Catalyst Dx Chemistry Analyzer system is a laboratory equipment used for the analysis of various chemical compounds in biological samples. The core function of this system is to perform automated clinical chemistry testing to support veterinary diagnosis and treatment.
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Lab products found in correlation
2 protocols using catalyst dx chemistry analyzer system
1
Serum Biomarker Profiling
2
Cardiac Puncture Blood Sampling and Metabolite Analysis
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Blood was collected via cardiac puncture and incubated at room temperature (23.0–25.2 °C) for 20 min in a BD Microtainer Serum Separator Tube (BD Biosciences). After centrifugation at 3000×g for 20 min, blood urea nitrogen and creatinine levels in the serum samples were measured using a Catalyst Dx Chemistry Analyzer system (IDEXX Laboratories, Inc., Westbrook, Maine).
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