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2 protocols using l casei

1

Cultivation of Diverse Microbial Strains

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A. oryzae ATCC42149, S. cereviceae ATCC9763, L. casei ATCC334, L. acidophillus ATCC314, L. fermentum ATCC23271, L. rhamnosus GG (LGG) ATCC53103, B. infantis ATCC15697, B. lactis ATCC700541, B. longum ATCC15707 and B. adlescentis ATCC15703 were purchased from ATCC. Fungi (A. oryzae, S. cereviceae), Lactobacilli (L. casei, L. acidophillus, L. fermentum, LGG) and Bifidobacterium (B. infantis, B. lactis, B. longum, B. adlescentis) were cultured in YM broth (Difco Laboratories, Detroit, MN, USA), Man–Rogosa–Sharpe (MRS) broth (Difco Laboratories) and Clostoridial broth (Difco Laboratories), respectively, for 2–3 day at 37 °C. Each of the Lactobacilli was then cultured in DMEM for another day.
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2

Evaluation of Probiotic Bacterial Strains

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The bacterial species evaluated in this study were B. animalis (from ACTIVIA ® ), B. longum, (ATCC 15707), B. lactis (LMG 18905), and B. dentium (ATCC 27678); L. acidophilus (ATCC 4356), L. casei (ATCC 393); S. mutans (ATCC 25175 and 3VF2), and S. sobrinus (ATCC 27607). All ATCC strains were obtained from the Oswaldo Cruz Foundation (FIOCRUZ, Rio de Janeiro, RJ, Brazil), and the André Tosello Foundation (Campinas, SP, Brazil). S. mutans 3VF2 is a highly acidogenic clinical strain that was previously characterized and also provided by Dr. Renata de Oliveira Mattos-Graner (FOP-UNICAMP, Piracicaba, Brazil). 9 B. animalis was isolated from ACTIVIA ® yogurt. Bifidobacterium, Lactobacillus, and Streptococcus species were isolated from the following media: transgalactosylated oligosaccharides propionate agar supplemented with lithium mupirocin (50 mg/L) (TOS-MUP agar; Merck Millipore, Darmstadt, Germany), Rogosa Agar (Difco Laboratories, Detroit, USA) and Mitis Salivarius Agar (MSA, Difco), respectively. 7 (link) All bacteria were grown at 37 o C for 48 h in a 5% CO 2 atmosphere.
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