Plasma samples from patients enrolled in both DOSE-AHF and CARRESS-HF were collected a baseline and at 72 hours (DOSE-AHF) or 96 hours (CARRESS-HF) and analyzed at a central core laboratory blinded to treatment assignment. RAAS activation was characterized by serum plasma renin activity (PRA) and aldosterone. PRA was measured by GammaCoat Plasma Renin Activity 125 I Radioimmunoassay Kit (catalog #CA-1533) by Diasorin (Stillwater, MN). Expected values for PRA are 0.85-16.34 ng/mL/hr and the inter-assay CV provided by the manufacturer is less than 10%. Aldosterone was measured by a radioimmunoassay manufactured by Diasorin (catalog #ALDOCTK-2). Expected values for Aldosterone in normal, healthy individuals are 7.5-150 pg/mL. The inter-assay CV provided by the manufacturer is less than 5.3%.
Gammacoat plasma renin activity 125 1 radioimmunoassay kit
Manufactured by DiaSorin
Sourced in United States
The GammaCoat Plasma Renin Activity 125 I Radioimmunoassay Kit is a laboratory diagnostic product designed to quantitatively measure the levels of renin activity in plasma samples. It utilizes a radioimmunoassay technique with a 125I-labeled tracer to detect and quantify the target analyte.
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Lab products found in correlation
2 protocols using gammacoat plasma renin activity 125 1 radioimmunoassay kit
1
RAAS Activation in Heart Failure Patients
2
Quantification of Mast Cell-Derived Renin
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Determination of mast cell-derived renin has been used as an additional suitable marker of mast cell degranulation induced by I/R. Thus, as a further test of potential inhibitory effects of 4CPI on mast cell degranulation, we evaluated a possible reduction of mast cell-derived renin in 4CPI-treated hearts submitted to I/R.
Renin activity was measured in samples collected from coronary effluent using a GammaCoat Plasma Renin Activity 125I Radioimmunoassay kit (DiaSorin, Stillwater, MN, USA) according to the manufacturer's instructions. Briefly, 200 l each sample of coronary effluent were incubated with porcine angiotensinogen (0.1 mg/ml), maleate buffer and PMSF (part of the DiaSorin kit). The reaction for angiotensin I production was conducted for 1.5 h at 37 • C. Results were normalized to total heart weight. The detection limit was approximately 0.01 pmol.
Renin activity was measured in samples collected from coronary effluent using a GammaCoat Plasma Renin Activity 125I Radioimmunoassay kit (DiaSorin, Stillwater, MN, USA) according to the manufacturer's instructions. Briefly, 200 l each sample of coronary effluent were incubated with porcine angiotensinogen (0.1 mg/ml), maleate buffer and PMSF (part of the DiaSorin kit). The reaction for angiotensin I production was conducted for 1.5 h at 37 • C. Results were normalized to total heart weight. The detection limit was approximately 0.01 pmol.
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