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N nornuciferine

Manufactured by Yuanye Bio-Technology
Sourced in China

N-nornuciferine is a laboratory compound used for research purposes. It is a heterocyclic organic molecule that is commonly utilized in various analytical and experimental procedures within scientific research settings. The core function of N-nornuciferine is to serve as a chemical tool for investigators, though its specific applications may vary depending on the research context.

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3 protocols using n nornuciferine

1

Standardization of Alkaloidal Compounds

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Coclaurine (wkq20071007) was purchased from Sichuan Victory Biological Technology Co., Ltd. (Sichuan, China). N-norNuciferine (B21944) was obtained from Shanghai Yuanye Bio-technology Co., Ltd. (Shanghai, China). Neferine (BCY-000435), liensinine (BCY-000438), armepavine (BCY-001299), and isoliensinine (250117-201812) were procured from Jiangxi Baicaoyuan Bio-technology Co., Ltd. (Jiangxi, China). Nuciferine (CFN99733) was purchased from Chemfaces. Acetonitrile, methanol, and formic acid (high-performance liquid chromatography grade) were obtained from Sigma-Aldrich Corporation (St. Louis, MO, USA). Ultrapure water was prepared using a Mill-Q SP system (Millipore Co., Bedford, MA, USA).
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2

Analytical Standards for Alkaloid Analysis

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HPLC-grade acetonitrile and methanol were purchased from Merck (Darmstadt, Germany), and HPLC-grade formic acid was obtained from Aladdin Reagent (Shanghai, China). Ethanol and ethyl lactate of analytical grade were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
Standards of alkaloids: nicotinamide (≥98%) and nuciferine (≥98%) were purchased from Aladdin Reagent (Shanghai, China). Asimilobine (≥98%) was purchased from Hubei Qifei Pharmaceutical and Chemical Co., Ltd. (Tianmen, China). N-nornuciferine (≥98%), N-methylcoclaurine (≥95%) and coclaurine (≥98%) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). A mixed standard of cis-N-feruloyltyramine (≥80%) and trans-N-feruloyltyramine (≥18%) was purchased from Shanghai Chengshao Biological Technology Co., Ltd. (Shanghai, China). Isoliensinine and neferine were isolated from Nelumbo nucifera Gaertn. extracts according to the experimental method reported in [14 (link)].
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3

Alkaloid Extraction and Quantification

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Alkaloid extraction and separation methods used
herein were the same as those described in a previous study.5 (link) Separation and detection were performed by HPLC-DAD
(SHIMADZU) using a TOSOH C18 column (4.6 mm × 150 mm, 3.5 μm
diameter). Nuciferine and N-norNuciferine alkaloid
contents were determined against the linear standard curves of Nuciferine
(Yuanye Biotechnology Shanghai, China) as a representative aporphine-type
alkaloid (Figure S2). The peak areas of
Nuciferine and N-norNuciferine were pooled to calculate
the total content of major BIAs.
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