Gabapentin was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). LPS (L2880) from Escherichia coli O127:B8 were obtained from Sigma–Aldrich Chemical Co. (St Louis, MO, USA). Anti-rabbit IL-1β and anti-mouse TNF-α antibodies were purchased from Abcam Inc. (Cambridge, MA, USA). Anti-rabbit p-cPLA2, cPLA2, COX-2 antibodies were purchased from Cell Signaling Technology (CST).
Gabapentin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Gabapentin is a chemical compound used as a raw material for the production of pharmaceutical products. It is a white, crystalline powder with a molecular formula of C₉H₁₇NO₂. Gabapentin is primarily used as a precursor in the synthesis of various medications, but its specific core function and intended use should not be extrapolated upon in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti tnf α, by Abcam (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Recombinant human macrophage colony stimulating factor m csf, by R&D Systems (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti flag, by Merck Group (1 mentions)
Trap staining kit, by Fujifilm (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti phospho jnk t183 y185, by Cell Signaling Technology (1 mentions)
Anti nfatc1, by Santa Cruz Biotechnology (1 mentions)
Anti iκbα, by Cell Signaling Technology (1 mentions)
Rankl, by R&D Systems (1 mentions)
4 protocols using gabapentin
1
Anti-inflammatory Signaling Pathways
2
TSP2 and Gabapentin Effects under High Pressure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified human recombinant TSP2 protein was purchased from R&D systems (1635-T2, Minnesota, USA) and gabapentin (GBP) from SANTA CRUZ (60142-96-3, Dallas, USA). All drugs were dissolved in sterile PBS as stock solutions and warmed to room temperature before performing experiments. TSP2, which was used at a concentration of 10 nM (~1.29 μg/ml), and gabapentin, which was used at a concentration of 32 μM (~5.47 μg/ml), were administered directly to cell cultures before high pressure treatment. The incubations were maintained for 14 h, including 2 h cultured in high pressure, and subsequently subjected to immunofluorescence and Western Blot.
3
Gabapentin Administration in Behavioral Experiments
4
Osteoclastogenesis Regulation by BCAAs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant human macrophage-colony stimulating factor (M-CSF) and RANKL were purchased from R&D systems (Minneapolis, MN, USA) and PeproTech EC Ltd. (London, UK) respectively. The TRAP staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation (Tokyo, Japan). Lipopolysaccharide (LPS), BCAAs (L-leucine, L-isoleucine, L-valine), and BCKAs [KIC (4-Methyl-2-oxovaleric acid), KMV ((±)-3-Methyl-2-oxovaleric acid sodium salt) and KIV (Sodium 3-methyl-2-oxobutyrate)] were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Gabapentin was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The following antibodies were used in western blotting: anti-ERK, anti-phospho-ERK (T202/Y204), anti-p38, anti-phospho-p38 (T180/Y182), anti-JNK, anti-phospho-JNK (T183/Y185), anti-AKT, anti-phospho-AKT (S473), anti-p65, anti-phospho-p65 (Y536), anti-IκBα (Cell Signaling Technology, Danvers, MA, USA), anti-β-Actin, anti-NFATc1 (Santa Cruz Biotechnology), anti-BCAT1 (Novus Biologicals, Centennial, CO, USA), anti-BCAT2 (Abcam, Cambridge, UK) and anti-FLAG (Sigma-Aldrich). The anti-Atp6v0d2 antibody was provided by Y. Choi (University of Pennsylvania, Philadelphia PA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!