The chemically synthesized TMEM9‐specific siRNA (5′‐GAATGACACAGCAATGAA‐3′) miR‐150‐5p mimics, miR‐150‐5p inhibitors and miRNA controls (miR‐NC) were purchased from GENECHEM (Shanghai, China). Using Lipofectamine 3000 (Invitrogen), TMEM9‐FLAG plasmid, TMEM9 siRNA, scrambled siRNA, miRNA mimics, miRNA inhibitors and miRNA controls were transfected into the cells according to the manufacturer's instructions.
Mirna controls mir nc
Manufactured by Genechem
Sourced in China
The miR-NC is a set of microRNA (miRNA) negative controls for use in miRNA expression analysis. It provides a non-targeting control miRNA sequence to help assess background signal in miRNA experiments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mirna controls mir nc
1
TMEM9 siRNA and miR-150-5p Modulation
2
Silencing Circular RNA and MicroRNA Transfection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For transfection with small interfering RNA (siRNA), negative control (NC) siRNAs, and siRNAs for hsa-circ-0012129 were obtained from GenePharma Co., Ltd (Shanghai, China). One day before transfection, U373 and SHG44 cells (2×104 cells/well) in the logarithmic phase were seeded into six-well plates in 2 mL of medium and incubated overnight. The next day, cells were transfected with 50 μM of scramble siRNA as the negative control (NC), and hsa-circ-0012129 siRNAs using Lipofectamine 3000 reagent (Invitrogen) (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol. After 48 hrs, the cells were harvested for further experiments.
For miRNA transfection, the miR-661 inhibitor (anti-miR-661) and miRNA controls (miR-NC) were purchased from Shanghai GeneChem Co., Ltd. (Shanghai, China). U373 cells were seeded in six-well plates (1×105 cells/well) and were transfected with anti-miR-661 and miR-NC using Lipofectamine 3000 reagent (Invitrogen) (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol.
For miRNA transfection, the miR-661 inhibitor (anti-miR-661) and miRNA controls (miR-NC) were purchased from Shanghai GeneChem Co., Ltd. (Shanghai, China). U373 cells were seeded in six-well plates (1×105 cells/well) and were transfected with anti-miR-661 and miR-NC using Lipofectamine 3000 reagent (Invitrogen) (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!