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3 protocols using 2 hydroxyethyl methacrylate 2 hema

1

Nephrite-Based Drug Delivery System

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Nephrite was obtained from Sujenara Co. (Seoul, Korea). Dopamine, ciprofloxacin HCl (CIP), levofloxacin (LEVO), ethylene glycol dimethacrylate (EGDMA), azobisisobutyronitrile (AIBN), 2-hydroxyethyl methacrylate (2-HEMA) and tryptic soy broth (TSB) were purchased from Sigma-Aldrich Chem. Co. Ltd. (St. Louis, MO, USA). Dialysis membranes with 500~1000 g/mol and 8000 g/mol of molecular weight cut-off (MWCO) were purchased from Spectrum Lab., Inc. (New Brunswick, NJ, USA). All of the organic solvents, such as dichloromethane (DCM), dimethylsulfoxide (DMSO) and ethyl alcohol (EtOH), were used as an ultrapure grade.
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2

Antimicrobial Polymer Synthesis and Evaluation

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Rifampicin (MW 822.95 amu) was purchased from TGI (Tokyo, Japan), cefixime trihydrate (MW 507.5 amu) was kindly donated by Dar Al Dawa Pharmaceuticals (Amman, Jordan). 2-Hydroxyethyl methacrylate (2-HEMA) (MW 130.14 amu), ethylene glycol di methacrylate (EGDMA) (MW 198.22 amu), 2.2-Azobis (2-methyl-propionitrile) (AIBN) (MW 164.21 amu) were obtained from Sigma-Aldrich (Munich, Germany). Bacteria were obtained from American Type Culture Collection Organization (Manassas, Virginia, USA); Staphylococcus aureus (S. aureus) ATCC 6538, Escherichia coli (E. coli) ATCC 8739, and Pseudomonas aeruginosa (P. aeruginosa) ATCC 9027. Brain Heart Infusion broth (BHI) was purchased from biolab (Budapest, Hangary). Müller Hinton agar and Nutrient Broth (NB) obtained from HIMEDIA (Mumbai, India). Human embryonic kidney (HEK) cell line from ATCC (USA).
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3

Antimicrobial Coating for Medical Devices

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The coating components: ethylene glycol dimethacrylate (EGDMA), 2-hydroxy-4′-(2hydroxyethoxy)-2-methylpropiophenone, 2-hydroxyethyl methacrylate (2-HEMA), chlorhexidine and isopropanol; the artificial urine components: potassium dihydrogen orthophosphate, magnesium chloride hexahydrate, urea, calcium chloride dihydrate and chicken ovalbumin; and the neutralizer components: Tween 80 and azolectin, were obtained from Sigma-Aldrich (Dorset, UK). Kollidon 90F was supplied by BTC Europe (Germany). Poly(vinyl chloride) (PVC) sheets (unplasticised, 0.2 mm thickness) were purchased from Goodfellow Ltd. (Cambridge, UK). Phosphatebuffered saline (PBS), quarter-strength Ringer's solution (QSRS), tryptone soya broth (TSB), agar, glycerol and Mueller-Hinton broth (MHB) were obtained from Oxoid Ltd.
(Hampshire, UK). Proteus mirabilis ATCC 35508 and Staphylococcus aureus ATCC 6538 (LGC Standards, Middlesex, UK) were maintained on cryopreservative beads (Protect Bacterial Preservation System, Technical Service Consultants Ltd., UK) in 10% glycerol at -80C and cultured by inoculation into MHB and incubation at 37C when required for the in vitro microbiological assessments.
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