Lc3 1 2

Manufactured by GeneTex

Sourced in United States

LC3 I/II is a laboratory equipment product that functions as a marker for autophagy. It is a widely used tool in the scientific community for the detection and analysis of autophagy-related processes.

Automatically generated - may contain errors

Lab products found in correlation

P ikk, by Cell Signaling Technology (1 mentions) Catalase, by GeneTex (1 mentions) Caspase 12, by GeneTex (1 mentions) Anti p38, by Abcam (1 mentions) Anti nrf2, by Abcam (1 mentions) Anti ho 1, by Abcam (1 mentions) P erk, by GeneTex (1 mentions) Anti pparγ, by Abcam (1 mentions) Anti iκbα, by Abcam (1 mentions) Anti pi3k, by Merck Group (1 mentions) Anti p akt, by Merck Group (1 mentions) P jnk, by GeneTex (1 mentions) P lkb1, by GeneTex (1 mentions) Keap1, by GeneTex (1 mentions) Anti nf κb, by Abcam (1 mentions) Cox 2, by GeneTex (1 mentions) P mtor, by Cell Signaling Technology (1 mentions) Anti inos, by Abcam (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

3 protocols using lc3 1 2

Hispolon Inhibits Inflammatory Pathways

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Hispolon was acquired from BJYM Pharmaceutical. & Chemical Co., Ltd. (Beijing, China). The purity of hispolon was higher than 95% (Figure 1A). LPS, dexamethasone (DEX) and other chemicals, solvents, and reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA). Enzyme-linked immunosorbent assay (ELISA) kits for the determination of cytokine secretion were acquired from BioLegend Inc. (San Diego, CA, USA). Anti-PI3k and Anti-p-AKT were obtained from Merck Millipore (Merck KGaA, Darmstadt, Germany). The antibodies against TLR4, AKT, p-JNK, ATF6, p-CaMKK2, p-LKB1, catalase, GPx, SOD, Keap1, COX-2, caspase 12, IRE1, GRP78, PERK, CHOP, Beclin 1 and LC3 I/II were obtained from GeneTex (Irvine, CA, USA). Antibodies against IKK, p-IKK, JNK, p-ERK, ERK, p-p38, mTOR and p-mTOR were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-iNOS, anti-HO-1, anti-Nrf-2, anti-PPARγ, anti-IκBα, anti-NF-κB, anti-p38, and anti-β-actin were purchased from Abcam (Cambridge, UK). Determination of protein concentration using a Bio-Rad protein assay kit (Bio-Rad Laboratories Ltd., Watford, UK).

Huang C.Y., Deng J.S., Huang W.C., Jiang W.P, & Huang G.J. (2020). Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy. Nutrients, 12(6), 1742.

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Western Blot Analysis of Autophagy and Apoptosis Markers

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The electrophoresis and transfer methods were described in our previous study [23 (link)]. Membranes were incubated with primary anti-MMP-2/9 (GeneTex, CA, USA), ATG5/12 (Abcam, CB, UK), Beclin 1 (Cell Signaling, MA, USA), LC3-I/II (GeneTex, CA, USA), cleaved-caspase-3 (Abcam, CB, UK), cleaved poly (ADP-ribose) polymerase (PARP; Cell Signaling, MA, USA), anti-β-actin (Merck, Darmstadt, Germany), and anti–glyceraldehyde 3-phosphate dehydrogenase (Abcam, CB, UK) antibodies at 4 °C overnight. Furthermore, they were incubated with the appropriate horseradish peroxidase-conjugated anti-rabbit antibody (Cell Signaling, MA, USA) or anti-mouse antibody (Sigma-Aldrich/Merck KGaA, Darmstadt, Germany) at 37 °C for 1 h. Enhanced chemiluminescence was then added to the blots, which were imaged using a ChemiDoc-It Imaging System (UVP Inc., CA, USA).

Hwang T.I., Chen P.C., Tsai T.F., Lin J.F., Chou K.Y., Ho C.Y., Chen H.E, & Chang A.C. (2022). Hsa-miR-30a-3p overcomes the acquired protective autophagy of bladder cancer in chemotherapy and suppresses tumor growth and muscle invasion. Cell Death & Disease, 13(4), 390.

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Western Blot Analysis of Cardiac Proteins

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Protein samples (50 mg) were separated in SDS-PAGE and transferred onto a nitrocellulose membrane. The blots were blocked with 5% nonfat milk for 2 h at room temperature, then incubated with primary antibody including BNP (1:1,000 dilution, sc-271185; Santa Cruz), β-MHC (1:2,000 dilution, SAB2106550; Sigma), Beclin-1 (1:1,000 dilution, ab207612; Abcam), LC3-II/I (1:500 dilution, GTX100240; GeneTex), p62 (1:1,000 dilution, 5114S; CST), P-AMPK (1:500 dilution, bs-4002R; Bioss), T-AMPK (1:1,000 dilution, E-AB-33742; Elabscience), P-mTOR (1:500 dilution, bs-3494R; Bioss), T-mTOR (1:200 dilution, BM4182; Boster), PPARγ (1:500 dilution, bs-4590R; Bioss), Nuclear NF-κB (1:500 dilution, bs-0465R; Bioss), total NF-κB (1:200 dilution; Abcam), GAPDH (1:2000; Zhong Shan-Golden Bridge Biological Technology), and β-actin (1:5,000 dilution; Santa Cruz), respectively, at 4°C overnight. The western blot bands were collected by Imaging System (LI-COR Biosciences) and quantified with Odyssey v.1.2 software by measuring intensity (area × optical density [OD]) in each group with β-actin as the internal control.

Qi H., Ren J., Ba L., Song C., Zhang Q., Cao Y., Shi P., Fu B., Liu Y, & Sun H. (2019). MSTN Attenuates Cardiac Hypertrophy through Inhibition of Excessive Cardiac Autophagy by Blocking AMPK /mTOR and miR-128/PPARγ/NF-κB. Molecular Therapy. Nucleic Acids, 19, 507-522.

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