Tissues were lysed using mortar and pestle, resuspended in RIPA buffer (1% Triton, 1% deoxycholate, 0.1% SDS, 0.16M NaCl, 10 mmol/L Tris pH 7.4, and 5 mmol/L EDTA), supplemented with a protease inhibitor cocktail (Pharmingen, San Diego, CA, USA), and analyzed as described previously (48). Primary antibodies used for DEK were as follows: DEK (1:1000; BD Biosciences, San Diego, CA, USA), pan-actin (1:20,000; a gift from James Lessard). Membranes were exposed to enhanced chemiluminescence reagents (Perkin Elmer, Boston, MA, USA) and imaged using the BioRad Chemidoc (Hercules, CA, USA).
Pan actin
Manufactured by BD
Sourced in United States
Pan-actin is a high-quality laboratory reagent used for the detection and quantification of actin, a ubiquitous cytoskeletal protein found in eukaryotic cells. The product provides a reliable, specific, and sensitive method for analyzing actin expression and distribution in various cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pan actin
1
Western Blot Protein Analysis Protocol
2
Modulation of Nitric Oxide Signaling
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NG-nitro-L-arginine methyl ester (L-NAME) and manganese (III) tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP) were purchased from Calbiochem; Merck KGaA. Anti-sera against eNOS, phospho-eNOS (Ser1177 and Thr495), phospho-CaMKII, phospho-AKT (Ser473) and pan-actin were obtained from BD Biosciences, and antiserum to p32 was obatined from Abcam. The siRNA against arginase II (siArgII; cat. no. sc-29729) and scrambled RNA (scm; cat. no. sc-37007) were purchased from Santa Cruz Biotechnology, Inc. All other chemicals were obtained from Sigma-Aldrich, Merck KGaA, unless otherwise stated.
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