Wildtype (N2) and atfs-1(et18) worms were synchronized by bleaching, and harvested at the L4 stage of development. Total RNA was extracted using the RNA STAT reagent (Tel-Test Inc.) and used for double-stranded cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories). Microarray analysis using GeneChip C. elegans genome arrays (Affymetrix) was conducted as previously described4 (link). Differences in gene expression between wildtype and atfs-1(et18) worms was determined using Anova streamlined (Partek Genomic Suite (v6.5)). Supplementary Table 1 contains the relative fold induction and p values for each mRNA. Confirmation of the microarray results was performed via qRT-PCR as described4 (link). Primers are listed in Supplementary Table 2 .
Genechip c elegans genome arrays
Manufactured by Thermo Fisher Scientific
The GeneChip C. elegans genome arrays are a type of microarray used for analyzing gene expression in the model organism Caenorhabditis elegans. These arrays contain probes designed to detect and quantify the expression levels of C. elegans genes. The arrays provide a comprehensive and unbiased approach to studying the transcriptome of this widely used model organism.
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Lab products found in correlation
3 protocols using genechip c elegans genome arrays
1
Transcriptional Profiling of atfs-1 Mutant C. elegans
2
Transcriptomic Analysis of Hsp90-depleted C. elegans
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After RNAi treatment for roughly 2.5 days nematodes were harvested for DNA microarray experiments. Hsp90-depleted and control vector treated nematodes were washed off the plates and collected in 15 mL plastic tubes. Nematodes were separated from residual bacteria by gravity sedimentation. The worms were frozen at -80 °C. RNA extraction and analysis of the mRNA levels was performed at the Kompetenzzentrum für Fluoreszente Bioanalytik (KFB) as a for-fee service. For this analysis Affymetrix GeneChip C. elegans Genome Arrays were used and the data for all genes were RMA-normalized. All microarray datasets will be uploaded to the GEO repository.
3
Transcriptional Profiling of atfs-1 Mutant C. elegans
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Wildtype (N2) and atfs-1(et18) worms were synchronized by bleaching, and harvested at the L4 stage of development. Total RNA was extracted using the RNA STAT reagent (Tel-Test Inc.) and used for double-stranded cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories). Microarray analysis using GeneChip C. elegans genome arrays (Affymetrix) was conducted as previously described4 (link). Differences in gene expression between wildtype and atfs-1(et18) worms was determined using Anova streamlined (Partek Genomic Suite (v6.5)). Supplementary Table 1 contains the relative fold induction and p values for each mRNA. Confirmation of the microarray results was performed via qRT-PCR as described4 (link). Primers are listed in Supplementary Table 2 .
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