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Azithromycin

Manufactured by Bioanalyse
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Azithromycin is a macrolide antibiotic used in the laboratory setting. It is a white crystalline powder and functions as an antimicrobial agent.

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Lab products found in correlation

Amikacin, by Bioanalyse (3 mentions) Vancomycin, by Bioanalyse (3 mentions) Methicillin, by Bioanalyse (3 mentions) Tetracycline, by Bioanalyse (3 mentions) Kanamycin, by Bioanalyse (3 mentions) Penicillin g, by Bioanalyse (3 mentions) Oxacillin, by Thermo Fisher Scientific (2 mentions) Amoxicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Rifampicin, by Thermo Fisher Scientific (2 mentions) Carbenicillin, by Thermo Fisher Scientific (2 mentions) Ampicillin, by Thermo Fisher Scientific (2 mentions) Erythromycin, by Thermo Fisher Scientific (2 mentions) Ofloxacin, by Thermo Fisher Scientific (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Amoxicillin clavulanic acid, by Bioanalyse (1 mentions) Mueller hinton agar (mha), by Thermo Fisher Scientific (1 mentions) Cefotaxime, by Thermo Fisher Scientific (1 mentions) Trimethoprim sulfamethoxazole, by Bioanalyse (1 mentions) Antibiotic discs, by Bioanalyse (1 mentions)

5 protocols using azithromycin

1

Antimicrobial Susceptibility of CR-GNB

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All isolates underwent antimicrobial susceptibility testing using cefotaxime (30 μg), gentamicin (10 μg) (Oxoid, UK), ofloxacin (5 μg), azithromycin (15 μg), amoxicillin-clavulanic acid (30 μg), trimethoprim-sulfamethoxazole (25 μg) (Bioanalyse, Turkey), imipenem (10 μg), meropenem (10 μg), ertapenem (10 μg), and doripenem (10 μg) (Lilofilchem, Italy), according to the Clinical and Laboratory Standards Institute (CLSI) guidelines (12 (link)) by the disc diffusion method using Mueller–Hinton agar (MHA) (Oxoid). Escherichia coli ATCC 25922 and P. aeruginosa ATCC 27853 were used as quality control strains.
40 selected CR-GNB isolates (P. aeruginosa (n=14, 35%), Enterobacter spp. (n=12, 30%), Klebsiella oxytoca (n=8, 20%), E. coli (n=4, 10%) and Citrobacter koseri (n=2, 5%)) for use in further investigations.
Fawzy R.H., Gad G.F, & Mohamed H.A. (2022). Phenotypic and genotypic detection of resistance mechanisms in carbapenem-resistant gram-negative bacteria isolated from Egyptian ICU patients with first emergence of NDM-1 producing Klebsiella oxytoca. Iranian Journal of Microbiology, 14(6), 832-840.
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2

Methicillin Resistance Detection in Staphylococcus

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The disc diffusion technique was used to test all staphylococcal isolates for methicillin resistance. PCR verified methicillin resistance to detect the mecA gene.16 (link) The isolates' susceptibility was evaluated using the disc diffusion technique20 using the following antibiotic discs (Bioanalyse, Turkey): Amikacin AK 30 μg, Azithromycin AZM 15 μg, Clindamycin CD 2 μg, Ciprofloxacin CIP 5 μg, Erythromycin E 15 μg, Gentamicin G 10 μg, Levofloxacin LEV 5 μg, Norfloxacin NOR 10 μg, Penicillin P 10 U, Tetracycline TE 30 μg, Trimethoprim+Sulfamethoxazole SXT 1.25+23.75 μg, and Vancomycin VA 30 μg. S. aureus ATCC 25923 was used as the control strain.
, & Hamad P.A. (2023). Phenotypic and Molecular Detection of Biofilm Formation in Methicillin-Resistant Staphylococcus Aureus Isolated from Different Clinical Sources in Erbil City. Mediterranean Journal of Hematology and Infectious Diseases, 15(1), e2023016.
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3

Antibiotic Susceptibility of L. nagelii

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Antibiogram tests were performed to screen resistance or sensitivity of AGA58 against commonly used antibiotics. Commercially available antibiotic disks [methicillin, vancomycin, amikacin, kanamycin, azithromycin, tetracycline, penicillin G (Bioanalyse, Yenimahalle, Ankara, Turkey); ampicillin, oxacillin, carbenicillin, amoxicillin, streptomycin, erythromycin, rifampicin (Oxoid, Basingstoke, Hampshire, UK)] were utilized to determine antibiotic susceptibility of L. nagelii AGA58 . The disk diffusion assay was performed per modified Kirby-Bauer method [29] (link). Interpretation of inhibition zone (mm) results was performed per Clinical and Laboratory Standards Institute's performance standards for antimicrobial testing [30] .
Inhibition zone less than or equal to 14 mm was noted resistant (R). Inhibition zone greater than 20 mm was regarded sensitive (S). Zones between 15 and 19mm were recorded as semi-sensitive or intermediate (I).
Yetiman A.E., & Ortakcı F. (2021). Metabolic potentials of Liquorilactobacillus nagelii AGA58 isolated from Shalgam based on genomic and functional analysis.
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4

Antibiotic Resistance Profiling of L. plantarum

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Antibiogram assays were conducted to nd out the resistance or sensitivity of the DY46 strain against commonly used antibiotics. Ready to use commercial antibiotic disks (methicillin, vancomycin, amikacin, kanamycin, azithromycin, tetracycline, penicillin G (Bioanalyse); ampicillin, oxacillin, carbenicillin, amoxycillin, streptomycin, erytromycin, rifampacin (oxoid)) were employed for antibiotic susceptibility testing of L. plantarum DY46. The application of disk diffusion assay was performed according to a modi ed Kirby-Bauer method (Sharma et al. 2017 (link)). Interpretation of inhibition zone (mm) results was carried out with regard to Clinical and Laboratory Standards Institute's performance standards for antimicrobial testing (CLSI M100-S22, 2012). Results with an inhibition zone less than or equal to 14mm were noted resistant (R). Additionally, inhibition zones greater than 20 mm were considered sensitive (S) and between 15-19 mm were accepted as semi-sensitive or intermediate (I).
Yetiman A.E., Keskin A., Kiraz B.N.D., Kotil S., Ortakcı F., & Doğan M. (2021). Characterization of genomic, physiological, and probiotic features of Lactiplantibacillus plantarum DY46 strain isolated from Turkish fermented turnip juice (Shalgam).
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5

Antibiotic Susceptibility of L. nagelii

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Antibiogram tests were performed to screen resistance or sensitivity of AGA58 against commonly used antibiotics. Commercially available antibiotic disks [methicillin, vancomycin, amikacin, kanamycin, azithromycin, tetracycline, penicillin G (Bioanalyse, Yenimahalle, Ankara, Turkey); ampicillin, oxacillin, carbenicillin, amoxicillin, streptomycin, erythromycin, rifampicin (Oxoid, Basingstoke, Hampshire, UK)] were utilized to determine antibiotic susceptibility of L. nagelii AGA58 . The disk diffusion assay was performed per modified Kirby-Bauer method [29] (link). Interpretation of inhibition zone (mm) results was performed per Clinical and Laboratory Standards Institute's performance standards for antimicrobial testing [30] .
Inhibition zone less than or equal to 14 mm was noted resistant (R). Inhibition zone greater than 20 mm was regarded sensitive (S). Zones between 15 and 19mm were recorded as semi-sensitive or intermediate (I).
Yetiman A.E, & Ortakci F. (2023). Genomic, probiotic, and metabolic potentials of Liquorilactobacillus nagelii AGA58, a novel bacteriocinogenic motile strain isolated from lactic acid-fermented shalgam. Journal of bioscience and bioengineering, 135(1).
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