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Ultrospec 6300 pro uv vis spectrophotometer

Manufactured by Cytiva
Sourced in United States, United Kingdom

The Ultrospec 6300 pro UV-vis spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of light through a sample over a range of ultraviolet and visible wavelengths. It is capable of analyzing the light absorption properties of various substances, providing quantitative data about the composition and concentration of samples.

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2 protocols using ultrospec 6300 pro uv vis spectrophotometer

1

Characterization of Graphene Quantum Dots

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The GQDs fluorescence spectra were obtained on a Cary Eclipse fluorescence spectrophotometer (Varian Co., USA). The absorption spectra of GQDs measured on the Ultrospec 6300 pro UV-vis spectrophotometer (Amersham Biosciences Co., USA) in a 1 cm quartz cells. A Fourier transform infrared (FT-IR) spectrophotometer (Thermo Scientific Nicolet iS5, USA) was used to collect the Fourier transform infrared spectra of GQDs. The morphology and size of GQDs were collected by transmission electron microscope (TEM) (JEM-2100, Japan), using an acceleration voltage of 200 kV.
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2

Characterization of PT-AuNS Nanoparticles

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The formation of PT-AuNS was examined using the Ultrospec 6300 pro UV–Vis spectrophotometer (Amersham Biosciences, Buckinghamshire, UK) in the wavelength range of 300–800 nm. The size, zeta potential, and PDI of PT-AuNS were determined using the Zetasizer Nano-ZS90 sample delivery system (Malvern Panalytical, Malvern, UK). The morphology and particle size distribution of PT-AuNS were determined using HR-TEM TALOS F200X (Thermo Scientific, Waltham, MA, USA) at an electron potential of 200 kV. To confirm that PT-AuNS were synthesized via PT extraction, FTIR spectra were obtained using the Spectrum GX spectrometer (Perkin Elmer Inc., Boston, MA, USA) prepared with potassium bromide pellets [47 (link)].
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