Cells were plated in a 96-well plate and cultured at 37 °C overnight. After that, cells were treated with etoposide at concentrations indicated in the figure legend. After 2 h incubation at 37 °C, the etoposide-containing medium was removed, and cells were washed with a medium. Cell culture was continued in a fresh medium at 37 °C for 72 h. Cell viability was measured using a Cell Counting Kit-8 (Dojindo, Japan) and a microplate reader (MPR-A100, AsOne, Japan).
Mpr a100
Manufactured by As One
Sourced in Japan
The MPR-A100 is a laboratory device designed for the measurement and analysis of various physical and chemical properties. It is a compact and versatile instrument that can be used in a wide range of research and testing applications.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 (cck8), by Dojindo Laboratories (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by As One (2 mentions)
Elisa pod substrate tmb kit, by Nacalai Tesque (1 mentions)
Immunowash 1575, by Bio-Rad (1 mentions)
Celltiter glo 3d cell viability assay reagent, by Promega (1 mentions)
2030 arvo x multilabel reader, by PerkinElmer (1 mentions)
7 protocols using mpr a100
1
Etoposide-Induced Cell Viability Assay
2
Cell Viability Assay with FNCs, GNPs, and FGNPs
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L929 or Colon26 cells were seeded on a 96-well plant at a density of 5.0 × 103 cells/well and incubated overnight. The cells were exposed to FNCs, GNPs, and FGNPs with varying concentration for 24 h. In the control, we added same volume of MilliQ (1 μL), which is used to prepare samples, to the cells. After addition of Cell Counting Kit-8, the cell viability was quantified by measuring absorbance at 450 nm and 650 nm (reference wavelength) using microplate reader (MPR-A100, ASONE).
3
SUPYN Monoclonal Antibody ELISA Protocol
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A SUPYN-specific monoclonal antibody14 (link) (clone: 2J16) was dissolved in carbonate buffer (0.5 µg/ml) and used as the capture antibody. 100 µl of the capture antibody solution was placed into wells on a 96-well plate and incubated overnight at 4 °C. Plates were washed 5 times with PBST (PBS with 0.05% Tween 20) using a microplate washer (ImmunoWash 1575:BioRad, Hercules, CA, USA) and blocked with 0.5% BSA / PBST for 1 h at room temperature. After washing, 50 μl of sample was diluted 1:2 with 25 μl of HAMA blocker (ab193969:abcam, Cambridge, UK) and 25 μl of PBS and dispensed into each well and incubated for 1 h at room temperature (RT). Plates were washed five times and HRP labeled anti-SUPYN monoclonal antibody (clone: 3H6) was used as a detection antibody at a concentration of 0.2 µg/ml. After 1 h at RT, plates were washed 5 times and incubated with 100 µl of POD (ELISA POD Substrate TMB kit: 05298-80: nakalai tesque, Kyoto, Japan) for 7 min at room temperature. Color development was stopped with 100 µl of H2SO4 and absorbance measured at a wavelength of 450 nm by a microplate reader (MPR-A100:ASONE, Osaka, Japan). ELISAs were performed in duplicate for each sample.
4
Colon26 Cell Viability Assay
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Murine colon cancer (Colon26) cells were seeded on 48 well plate (1.71 × 104 cells) and incubated 24 h. The cells were exposed to 1 which was complexed and solubilized with TMe-β-CDx at an optional concentration (0.0015–0.15 wt% 1 to cell culture medium). After an additional 24 h, cell counting kit-8 (CCK8) solution was added to each sample and the absorbance at 450 nm was measured using a microplate reader (MPR-A100, AS ONE, Tokyo, Japan) to quantify cell viability.
5
Evaluating Cell Viability via MTT Assay
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Initially, PI dye delivery (red fluorescence) and calcein-AM stained live cells (green fluorescence) were recorded by a fluorescence microscope. Then, the images were analyzed using the Image J software.52 The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, analytical grade, TCI, Japan) assay was accomplished using HeLa cells to measure the cell viability of all the tested samples, following the well-defined process in previous studies.12 ,53 Each sample was added to a 30 mm cell culture plate, and HeLa cells were seeded with a density of 0.5 × 105 cells per mL. The MTT assay was performed after 2 h, 3 days, and 7 days before and after laser exposure of the samples. For the assay, 50 μl of 5 mg ml–1 MTT (stock) was added in each plate and then incubated at a temperature of 37 °C in a 5% CO2 incubator for four hours, resulting in the formation of formazan crystals. Afterward, the cell culture medium containing MTT was removed from the culture plate by aspiration. Then, formazan crystals were dissolved by adding 500 μl dimethyl sulfoxide (DMSO, Sigma-Aldrich). Finally, 200 μl of dissolved formazan crystals with DMSO solution was transferred into a 96-well plate for the reading of optical density at 570 nm through a plate reader (AS ONE MPR-A 100).54
6
Cell Viability and ATP Measurement
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Cell viability was measured using a Cell Counting Kit-8 (Dojindo Laboratories, Japan) and a microplate reader (MPR-A100, AsOne, Osaka, Japan). Cell lysis and ATP measurement were carried out using a CellTiter-Glo 3D cell viability assay reagent (G9682, Promega, Madison, WI, USA) according to the manufacturer’s instructions. Luminescence was measured using a 2030 ARVO X multilabel reader (Perkin Elmer, Shelton, CT, USA).
7
Evaluating Cell Viability via MTT Assay
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