To induce chondrogenic and adipogenic differentiation, hPDLSCs was cultured in StemPro Chondrogenic and Adipogenic differentiation media (Gibco BRL), respectively, using appropriate nutritional supplements to induce cartilage and adipocyte formation. After 3 weeks, the differentiated hPDLSCs was fixed for 5 min using 4% paraformaldehyde solution and stained with 1% Alcian Blue (Sigma–Aldrich, for cartilage staining) and 0.3% Oil Red O dye (Sigma–Aldrich, for lipid droplet staining) Cells were observed using a microscope (Olympus, CKX53).
Stempro chondrogenic
Manufactured by Thermo Fisher Scientific
The StemPro Chondrogenic Differentiation Kit is a cell culture medium designed for the differentiation of mesenchymal stem cells (MSCs) into chondrocytes, the cells that make up cartilage. The kit provides the necessary growth factors and supplements to promote the chondrogenic lineage and support the development of a cartilage-like extracellular matrix.
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Lab products found in correlation
Oil red o dye, by Merck Group (2 mentions)
Alcian blue, by Merck Group (2 mentions)
Adipogenic differentiation media, by Thermo Fisher Scientific (1 mentions)
Ckx53, by Olympus (1 mentions)
Stempro adipogenic differentiation medium, by Thermo Fisher Scientific (1 mentions)
Alizarin red s stain, by Merck Group (1 mentions)
U spt, by Olympus (1 mentions)
2 protocols using stempro chondrogenic
1
Chondrogenic and Adipogenic Differentiation of hPDLSCs
2
Multilineage Differentiation of hPDLSCs
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To promote osteogenic, chondrogenic, and adipogenic differentiation, hPDLSCs were cultured in StemPro Osteogenic, StemPro Chondrogenic, and StemPro Adipogenic differentiation medium (Gibco BRL), respectively, with the appropriate supplements as previously reported [17 (link)]. At 21 days, the cells with post-osteogenic, post-chondrogenic, and post-adipogenic induction were stained with 2% Alizarin Red S stain at pH 4.2 (Sigma-Aldrich), 1% Alcian Blue (Sigma-Aldrich), and 0.3% Oil Red O dye (Sigma-Aldrich) to detect proteoglycans, Nissl bodies, and fat vacuoles as indicators of osteogenic, chondrogenic, and adipogenic differentiation, respectively. Stained cells were observed and those were visualized under an inverted light microscope (Olympus U-SPT; Olympus).
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