Droplets were gently transferred with a cut-off transfer pipette into EM fix: 0.15 M sodium cacodylate trihydrate (Sigma) dissolved in water (pH 7.3) containing 4% formaldehyde and 2% glutaraldehyde (Electron Microscopy Sciences), and stored overnight. Samples were post-fixed with osmium tetroxide solution (Sigma), dehydrated in serial ethanol dilutions (Sigma), and embedded in epoxy resin. Ultrathin sections (80 nm) were mounted on 200 mesh copper grids and stained with uranyl acetate and lead citrate (Electron Microscopy Sciences). Imaging was performed with a JEOL JEM-1010 TEM and a FEI Tecnai G2 Spirit TEM.
Jem 1010 tem
Manufactured by Thermo Fisher Scientific
The JEM-1010 TEM is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of samples. It provides a core function of magnifying and projecting an electron-transparent specimen onto a fluorescent screen or other imaging devices, enabling the observation of fine details and structures at the nanoscale level.
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Lab products found in correlation
2 protocols using jem 1010 tem
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Electron Microscopy Sample Preparation
2
Electron Microscopy Sample Preparation
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