Purasorb plc 7015

Purasorb PLC 7015 (PLCL with 70/30 L-lactide/ε-caprolactone proportion copolymer) was supplied by Corbion Purac (Amsterdam, The Netherlands). The melting temperature of the polymer is 107.3–112.4 °C. The polymer was stored in sealed bags in a freezer at −20 °C.
Analysis grade Sodium Chloride (NaCl) salt provided by Scharlab (Barcelona, Spain) was used as porogenerator. The salt was milled in a blender, sieved using 25, 50, and 100-µm mesh sizes, and kept in silica-gel desiccator at room temperature (RT).

Nanomaterials for our experiment were fabricated via electrospinning on the Nanospider^TM machine which is a construct of Technical University of Liberec, Faculty of Textile Enigineering, Department of Nonwovens and Nanofibrous Materials, Czech Republic. Electrospinning has been selected as a method for fabrication of planar fibrous scaffolds as this technique allows large scale production of nanofibers; our team has also wide experience with this technique³⁹ .
We used two types of biocompatible polymers: polycaprolactone (PCL), and a polylactic acid-polycaprolactone copolymer (PLCL). PCL (mean weight = 43000 g/mol, Polysciences) and PLCL (Purasorb PLC 7015, Corbion) were dissolved in chloroform, acetic acid and ethanol solution (8:1:1 volume fractions) to the final concentration of 16% and 10% respectively (concentrations allowing optimal electrospinning properties based on previous research^{41 (link)}). The solutions were electrospun onto a spun bond (nonwoven cloth underlay) for easy manipulation and application. The material has been sterilised using ethylene oxide (37 °C, Anprolene). This method has been tested before to prove its safety and frugality to the nanomaterial^{41 (link)}.