Milli q direct q 3uv system

LC analyses were carried out with a nano-LC system consisting of a DiNa S (KYA Technologies, Tokyo, Japan) as the pump, a CE-2070 (JASCO, Tokyo, Japan) as the UV detector, a CHEMINERT (Valco Instruments, Houston, TX) as the sample injector, and a Chemco capillary column conditioner Model 380-b (Chemco, Osaka, Japan) as the column oven. A Nexera Micros (Shimadzu, Kyoto, Japan) series was used for gradient LC separation. This system consisted of LC-Mikros (Shimadzu, Kyoto, Japan) as the pump, MU701 (GL Sciences, Tokyo, Japan) as the UV detector, a CHEMINERT as the sample injector, and CTO-20AC (Shimadzu, Kyoto, Japan) as the column oven. Acetonitrile, DI, sodium phosphate buffer, and Tris–HCl buffer were utilized as the mobile phases. DI was obtained by a Milli-Q Direct-Q 3UV system (Merck Millipore, Tokyo, Japan). Sodium dihydrogenphosphate dihydrate, disodium hydrogenphosphate, and tris(hydroxymethyl)aminomethane were purchased from Tokyo Chemical Industry (Tokyo, Japan) for buffer preparation. As samples, AMP, ADP, ATP, phenylphosphoric acid disodium salt, benzyl alcohol, benzyl amine, aniline, benzene, anisole, acetophenone, ethyl benzoate, benzoic acid, phenol, and sodium benzenesulfonate were purchased from Nacalai Tesque (Kyoto, Japan). A bare capillary column was treated with water and acetone, and then dried.