Male BALB/c mice (8-weeks-old) were injected i.p. with 30 μg R2agoTNF or scR2agoTNF-Fc. To investigate pharmacokinetics, blood was collected from the tail vein of mice at an appropriate interval after injection. Protein concentrations in plasma samples were measured by a human TNF ELISA kit (BD Biosciences) or human IgG ELISA kit (Bethyl Laboratories, Montgomery, TX, USA). The t½ and AUC were calculated by moment analysis.
Human tnf elisa kit
Manufactured by BD
Sourced in United States
The Human TNF ELISA kit is a laboratory equipment designed to detect and quantify the presence of tumor necrosis factor (TNF) in human samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of this cytokine.
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3 protocols using human tnf elisa kit
1
Pharmacokinetics of R2agoTNF in Mice
2
Cytokine Profiling in Infection
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Demographic data were collected for all the subjects. For each subject, 3.5 mL whole blood was collected and results of a complete blood count with differential cytokine concentration measurement were obtained. Measurements of serum C-reactive protein (CRP) and cytokine (interleukin [IL]-10, IL-6, and TNF-a) concentrations were performed using enzyme-linked immunosorbent assays (ELISAs): Human IL-10 ELISA Kit (BD OptEIA, Beckton-Dickinson, Franklin Lakes, NJ), Human TNF Elisa Kit (BD OptEIA), and Human IL-6 ELISA Kit (R&D Systems, Minneapolis, Minn) according to the manufacturers' instructions. Results of microbiological investigations were collected. The primary source of infection and the Acute Physiology and Chronic Health Evaluation II score (20) were also recorded.
3
Quantification of TNFα and IL6 levels
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TNFα and IL6 levels in the medium of cultured monocytes were measured using human TNF ELISA kit (BD Bioscience, San Jose, CA, USA) or human IL6 DuoSet ELISA (R&D System, Abingdon, UK), respectively. TNFα and IL6 in serum were determined using the Milliplex Human Adipocyte panel (Millipore, Amsterdam, The Netherlands) and measured on the Bio-Plex array reader and Bio-Plex software in accordance with the manufacturer's instructions.
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