Alizarin red s st1078

Resveratrol (purity ≥99%; catalog number: R5010) and dexamethasone (≥98%; D1756) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Antibodies against p-AMPK (1:1000 dilution; 50081), AMPK (1:1000; 5832), p-ACC (1:1000; 11818), and ACC (1:1000; 3676) were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against β-Actin (1:1000; sc-47778), SOD1 (1:1000; sc-271014), and SOD2 (1:1000; sc-133134) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against PGC-1ɑ (1:500; AB3242) were from Millipore (Burlington, MA, USA). SIRT3 (1:1000; WL03840), Runx2 (1:1000; WL03358) and receptor activator of nuclear factor-κB ligand (RANKL; 1:600; WL00285) were from Wanleibio (Shenyang, China). MTT (ST1537) and Alizarin Red S (ST1078) were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Minimum essential medium-alpha modification (α-MEM; 32571101), Dulbecco’s modified Eagle’s medium (DMEM; 10566016) and fetal bovine serum (10270–106) were purchased from Gibco (Carlsbad, CA, USA). TRIzol^® Reagent (15596026) was obtained from Thermo Fisher Scientific (Waltham, MA, USA).