Multi-T probe 1 was 5′-TTT GCT TGT TGC GCT TCT TGC TTT-NH2-3′, Multi-T probe 2 was 5′-NH2-GAT AGC TTT GCT TGT TGC GCT TCT TGC TTT -FAM-3′, Multi-T probe 3 was 3’-NH2-CTA TCG-5’. The probes were purchased from Sangon Biotech Company (Shanghai, China) and purified by HPLC. Mercuric nitrate (Hg(NO3)2) was purchased from Shandong West Asia Chemical Industry Co., Ltd. (Lin Yi, China) and zinc chloride (ZnCl2), Nickel Chloride (NiCl2), Potassium Chloride (KCl), Lead Acetate ((CH3COO)2Pb), Magnesium Chloride (MgCl2), and Manganese Chloride (MnCl2) were purchased from Suzhou Asia Pacific Chemical Glass Instrument Co., Ltd. (Suzhou, China) Cadmium (CdCl2), calcium chloride (CaCl2), cobalt chloride (CoCl2), ferrous sulfate (FeSO4), ferric chloride (FeCl3), sodium chloride (NaCl), chromium trichloride (CrCl3), barium chloride (BaCl2), silver chloride (AgCl) were purchased from Sinopharm Chemicals (Shanghai, China), N-hydroxysuccinimide (NHS) and 1-3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was purchased from Sigma Co., Ltd. (St. Louis, MO, USA) SYBR Gree+-n I was purchased from Xiamen Zhishan Biotechnology Co., Ltd. (Xiamen, China) The Synergy H4 Multiplate Reader was used for fluorescence detection and Origin 8.0 was used for data analysis.
Chromium trichloride
Manufactured by Sinopharm
Sourced in China
Chromium trichloride is an inorganic compound with the chemical formula CrCl3. It is a dark green crystalline solid that is soluble in water and various organic solvents. Chromium trichloride is commonly used as a precursor in the synthesis of other chromium compounds and as a catalyst in various chemical processes.
Automatically generated - may contain errors
3 protocols using chromium trichloride
1
Multi-T Probe Synthesis and Metal Detection
2
Synthesis and Characterization of Chromium(III) Malate
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The chromium (III) malate was synthesized according to the method described by Li et al. [28 (link)] in our research group. Chromium picolinate and chromium trichloride were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Cholesterol and sodium cholate hydrate were produced at Hubei Hongyun long biological technology Co. Ltd. and Henan zhengxing food additive co., Ltd, respectively. Streptozotocin(STZ) was obtained from Sigma Chemical Co. Distilled water was used throughout the whole experiments.
3
Chromium-based Compound Synthesis
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Chromium trichloride, malic acid, and chromium picolinate were bought in Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). The chromium malate was synthesized in our research group. Nitric and perchloric acid were purchased from Sinopharm Chemical Reagent Co., Ltd. Ultrapure water was used in this experiment.
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